TRIM5 alpha Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-76601

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human

Applications

Validated:

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1 mg/ml

View all TRIM5 alpha Antibodies »

Product Specifications

Immunogen

Antibody was raised against a synthetic peptide corresponding to amino acids near the mid-region of human TRIM5 alpha. The immunogen is located within amino acids 370 - 420 of TRIM5 alpha. Amino Acid Squence: NIEKNENYQPKYGY

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for TRIM5 alpha Antibody - BSA Free

Western Blot: TRIM5 alpha AntibodyBSA Free [NBP1-76601]

Western Blot: TRIM5 alpha AntibodyBSA Free [NBP1-76601]

Western Blot: TRIM5 alpha Antibody [NBP1-76601] - Human stomach (A), thymus (B), and uterus (C) cell lysate with TRIM5 alpha antibody at 2 ug /ml.
Immunocytochemistry/ Immunofluorescence: TRIM5 alpha Antibody - BSA Free [NBP1-76601]

Immunocytochemistry/ Immunofluorescence: TRIM5 alpha Antibody - BSA Free [NBP1-76601]

Immunocytochemistry/Immunofluorescence: TRIM5 alpha Antibody [NBP1-76601] - Immunofluorescence of TRIM5 alpha in Human Stomach cells with TRIM5 alpha antibody at 10 ug/mL.
Immunohistochemistry-Paraffin: TRIM5 alpha Antibody - BSA Free [NBP1-76601]

Immunohistochemistry-Paraffin: TRIM5 alpha Antibody - BSA Free [NBP1-76601]

Immunohistochemistry-Paraffin: TRIM5 alpha Antibody [NBP1-76601] - Human stomach using TRIM5 alpha antibody at 2 ug/ml.

Applications for TRIM5 alpha Antibody - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

10 ug/ml

Immunohistochemistry

2 ug/ml

Immunohistochemistry-Paraffin

2 ug/ml

Western Blot

2 ug/ml
Application Notes
A band at approximately 55 kDa can be detected in Western Blot.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: TRIM5 alpha

TRIM5 is a member of a broad family of otherwise unrelated proteins defined by the presence of a tripartite motif containing a RING domain, a B-box type 1, and a B-box type 2, followed by a coiled-coil region . TRIM5 has six alternately spliced isoforms, the longest of which is the α variant which also contains a carboxy-terminal B30.2 (SPRY) domain. Expression of TRIM5α variants from humans, rhesus monkeys, and African green monkeys enabled resistance to infection by various retroviruses including HIV-1, albeit at differing efficiencies. All TRIM5α variants could inhibit at least two different retroviruses, but not from those viruses isolated from the same species, suggesting that TRIM5α acts as a natural barrier to cross-species retrovirus transmission.

Alternate Names

EC 6.3.2, EC 6.3.2.-, RING finger protein 88, RNF88tripartite motif protein TRIM, TRIM5alpha, tripartite motif containing 5, tripartite motif protein TRIM5, tripartite motif-containing 5, tripartite motif-containing protein 5

Gene Symbol

TRIM5

UniProt

NP_149023 (Human)

Additional TRIM5 alpha Products

Product Documents for TRIM5 alpha Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for TRIM5 alpha Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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