TRIM5 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-2474

Novus Biologicals, part of Bio-Techne
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NB100-2474
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all TRIM5 Antibodies »

Product Specifications

Immunogen

Peptide with sequence CNIEKNENYQPK corresponding to internal region according to NP_149023.1.

Specificity

This antibody is expected to recognise isoform alpha (NP_149023.1) only.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for TRIM5 Antibody

Immunohistochemistry-Paraffin: TRIM5 Antibody [NB100-2474]

Immunohistochemistry-Paraffin: TRIM5 Antibody [NB100-2474]

Immunohistochemistry-Paraffin: TRIM5 Antibody [NB100-2474] (4ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.

Applications for TRIM5 Antibody

Application
Recommended Usage

Immunohistochemistry

3 - 6 ug/ml

Immunohistochemistry-Paraffin

3 - 6 ug/ml

Peptide ELISA

Detection limit 1:32000
Application Notes
WB: Preliminary experiments gave an approx. 37 kDa band in Lysates of human cell lines Daudi, Jurkat and MOLT4 after 0.3 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 56.3 kDa band according to NP_149023.1. The 37 kDa band was successfully blocked by incubation with the immunizing peptide. IHC-P: Human kidney shows cytoplasm staining in PCT.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: TRIM5

The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein localizes to cytoplasmic bodies. Its function has not been identified. Five alternatively spliced transcript variants for this gene have been described. However, the full length nature of two of the variants has not been determined.

Long Name

Tripartite Motif-containing 5

Alternate Names

RNF88

Entrez Gene IDs

85363 (Human)

Gene Symbol

TRIM5

UniProt

NP_149023.1 (Human)

Additional TRIM5 Products

Product Documents for TRIM5 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for TRIM5 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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