TRIM56 Antibody (PSH02-17)

Western Blot: TRIM56 Antibody (PSH02-17) [NBP3-33055]

Western Blot: TRIM56 Antibody (PSH02-17) [NBP3-33055] - Western blot analysis of TRIM56 on different lysates with Rabbit anti-TRIM56 antibody (NBP3-33055) at 1/1,000 dilution.

Lane 1: HeLa cell lysate
Lane 2: MCF7 cell lysate
Lane 3: A549 cell lysate
Lane 4: 293T cell lysate
Lane 5: A375 cell lysate
Lane 6: COS-1 cell lysate
Lane 7: C2C12 cell lysate
Lane 8: PC-12 cell lysate
Lane 9: Mouse lung tissue lysate
Lane 10: Rat lung tissue lysate

Lysates/proteins at 30 ug/Lane.

Predicted band size: 81.5 kDa
Observed band size: 88 kDa

Exposure time: 3 minutes 54 seconds;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33055) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.

Flow Cytometry: TRIM56 Antibody (PSH02-17) [NBP3-33055]

Flow Cytometry: TRIM56 Antibody (PSH02-17) [NBP3-33055] - Flow cytometric analysis of HeLa cells labeling TRIM56.

Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-33055, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).