Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Validated:

Human

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all TRIO Antibodies »

Product Summary for TRIO Antibody

Immunogen

The immunogen this antibody was made to, maps to a region between residue 1575 to 1625 of human Trio Rho Guanine Nucleotide Exchange Factor using the numbering given in entry NP_009049.2 (GeneID 7204).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for TRIO Antibody

Western Blot: TRIO Antibody [NBP2-32088]

Western Blot: TRIO Antibody [NBP2-32088]

Western Blot: TRIO Antibody [NBP2-32088] - Whole cell lysate (50 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-TRIO antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Immunoprecipitation: TRIO Antibody [NBP2-32088]

Immunoprecipitation: TRIO Antibody [NBP2-32088]

Immunoprecipitation: TRIO Antibody [NBP2-32088] - Detection of human TRIO by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-TRIO antibody NBP2-32088 used for IP at 3 ug per reaction. TRIO was also immunoprecipitated by 2 other rabbit anti-TRIO antibodies. For blotting immunoprecipitated TRIO, NBP2-32088 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Applications for TRIO Antibody

Application
Recommended Usage

Immunoprecipitation

2 - 5 ug/mg lysate

Western Blot

1:1000 - 1:5000
Application Notes
Western blot of lysates performed using standard western blot reagents and 3-8% SDS-PAGE.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: TRIO

TRIO - triple functional domain (PTPRF interacting)

Alternate Names

ARHGEF23, EC 2.7.11.1, FLJ42780, PTPRF-interacting protein, tgat, triple functional domain (PTPRF interacting), triple functional domain protein

Gene Symbol

TRIO

UniProt

NP_009049.2 (Human)

Additional TRIO Products

Product Documents for TRIO Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for TRIO Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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