Triosephosphate isomerase Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB300-994

Novus Biologicals, part of Bio-Techne
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NB300-994
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Canine

Cited:

Human

Predicted:

Bovine (100%). Backed by our 100% Guarantee.

Applications

Validated:

Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all Triosephosphate isomerase Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-LKPEFVDIINAKQ corresponding to C-Terminus according to NP_000356.1; NP_001152759.1; NP_001244955.1.

Specificity

This antibody is expected to recognise reported isoforms 1, 2 and 3 (NP_000356.1; NP_001152759.1, NP_001244955.1)

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Theoretical MW

26.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Triosephosphate isomerase Antibody

Western Blot: Triosephosphate isomerase Antibody [NB300-994]

Western Blot: Triosephosphate isomerase Antibody [NB300-994]

Western Blot: Triosephosphate isomerase Antibody [NB300-994] - Staining (0.001ug/ml) of HepG2 (A), HEK293 (B), HeLa (C) and Jurkat (D) cell lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
Immunohistochemistry-Paraffin: Triosephosphate isomerase Antibody [NB300-994]

Immunohistochemistry-Paraffin: Triosephosphate isomerase Antibody [NB300-994]

Immunohistochemistry-Paraffin: Triosephosphate isomerase Antibody [NB300-994] - Staining of paraffin embedded Human Liver. Antibody at 2 ug/mL. Steamed antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining.
Flow Cytometry: Triosephosphate isomerase Antibody [NB300-994]

Flow Cytometry: Triosephosphate isomerase Antibody [NB300-994]

Flow Cytometry: Triosephosphate isomerase Antibody [NB300-994] - Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
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Applications for Triosephosphate isomerase Antibody

Application
Recommended Usage

Flow Cytometry

10 ug/ml

Immunohistochemistry

2 - 4 ug/mL

Immunohistochemistry-Paraffin

5 ug/mL

Peptide ELISA

Detection limit 1:16000

Western Blot

0.001 - 0.01 ug/mL
Application Notes
Approx 26 kDa band observed in HEK293, HeLa, HepG2, Jurkat, Raw264.7. KNRK and MDCK lysates (calculated MW of 26.7kDa according to NP_000356.1).

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Triosephosphate isomerase

Triosephosphate isomerase (TIM) catalyses the reversible interconversion of G3P and DHAP. Only G3P can be used in glycolysis, therefore TIM is essential for energy production, allowing two molecules of G3P to be produced for every glucose molecule, thereby doubling the energy yield. Defects in TPI1 are the cause of triosephosphate isomerase deficiency (TPI deficiency) . TPI deficiency is an autosomal recessive disorder. It is the most severe clinical disorder of glycolysis. It is associated with neonatal jaundice, chronic hemolytic anemia, progressive neuromuscular dysfunction, cardiomyopathy and increased susceptibility to infection.

Alternate Names

EC 5.3.1.1, MGC88108, TIM, TPI, triosephosphate isomerase, Triose-phosphate isomerase, triosephosphate isomerase 1

Entrez Gene IDs

7167 (Human); 21991 (Mouse); 24849 (Rat)

Gene Symbol

TPI1

UniProt

P60174 (Human)

Additional Triosephosphate isomerase Products

Product Documents for Triosephosphate isomerase Antibody

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Certificate of Analysis

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Product Specific Notices for Triosephosphate isomerase Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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