Tripeptidyl-Peptidase I/TPP1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-36742

Novus Biologicals, part of Bio-Techne
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NBP1-36742
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Bovine (100%), Canine (100%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all Tripeptidyl-Peptidase I/TPP1 Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-TPSVIRKRYNLTSQD corresponding to internal region according to NP_000382.3.

Epitope

internal region

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Tripeptidyl-Peptidase I/TPP1 Antibody

Western Blot: Tripeptidyl-Peptidase I/TPP1 Antibody [NBP1-36742]

Western Blot: Tripeptidyl-Peptidase I/TPP1 Antibody [NBP1-36742]

Western Blot: Tripeptidyl-Peptidase I/TPP1 Antibody [NBP1-36742] - (0.3ug/ml) Analysis of Human Placenta lysate (35ug protein in RIPA buffer) with (B) and without (A) blocking with the immunising peptide. Primary incubation was 1 hour. Detected by chemiluminescence.

Applications for Tripeptidyl-Peptidase I/TPP1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:200

Peptide ELISA

Detection limit 1:4000

Western Blot

0.3 - 1 ug/ml
Application Notes
WB: Approx. 65 kDa band observed in human placenta lysates (calculated MW of 61.2 kDa band according to NP_000382.3).
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Tripeptidyl-Peptidase I/TPP1

This gene encodes a member of the sedolisin family of serine proteases. The protease functions in the lysosome to cleave N-terminal tripeptides from substrates, and has weaker endopeptidase activity. It is synthesized as a catalytically-inactive enzyme which is activated and auto-proteolyzed upon acidification. Mutations in this gene result in late-infantile neuronal ceroid lipofuscinosis, which is associated with the failure to degrade specific neuropeptides and a subunit of ATP synthase in the lysosome.

Alternate Names

CLN2, LINCL, LPIC, TPP1, TripeptidylPeptidase I

Entrez Gene IDs

1200 (Human); 12751 (Mouse)

Gene Symbol

TPP1

UniProt

NP_000382.3 (Human)

Additional Tripeptidyl-Peptidase I/TPP1 Products

Product Documents for Tripeptidyl-Peptidase I/TPP1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Tripeptidyl-Peptidase I/TPP1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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