I am searching (with difficulty) for an antibody against Trka which can be used in flow cytometry. Since I am planning to use it for bead sorting of live cells it needs to be for a part of the receptor which is on the outside of the cell. Please can you let me know if you can help me with this.

Name: TrkA Antibody (6B2) - BSA Free
Brand: Novus Biologicals
SKU: NBP1-47436

NBP1-47436, NB100-98815 and NB100-65266 all bind to the extracellular domain of Trka, however, none of them are guaranteed for use in flow cytometry. Our only flow cytometry guaranteed antibodies bind to the intracellular domain. This does not mean one of these antibodies won't work for flow, we just can not guarantee it. If you would be interested in testing this novel application on one of our Trka antibodies, please take a look at our Innovators Reward Program.

TrkA Antibody (6B2) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-47436

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG₁ Clone # 6B2

Format

BSA Free

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

View all TrkA Antibodies »

Product Specifications

Immunogen

Purified recombinant extracellular fragment of human TrkA (aa33-423) fused with hIgGFc tag expressed in HEK293 cell line.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁

Theoretical MW

87.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for TrkA Antibody (6B2) - BSA Free

Western Blot: TrkA Antibody (6B2)BSA Free [NBP1-47436]

Western Blot: TrkA Antibody (6B2) [NBP1-47436] - Analysis using TrkA mouse mAb against extracellular domain of human TrkA(aa33-423).

Immunocytochemistry/ Immunofluorescence: TrkA Antibody (6B2) - BSA Free [NBP1-47436]

Immunocytochemistry/Immunofluorescence: TrkA Antibody (6B2) [NBP1-47436] - Analysis of PC-12 cells using TrkA mouse mAb (green), showing membrane and cytoplasmic localization. Blue: DRAQ5 fluorescent DNA dye.

Applications for TrkA Antibody (6B2) - BSA Free

Application

Recommended Usage

ELISA

1:10000

Immunocytochemistry/ Immunofluorescence

1:200 - 1:1000

Western Blot

1:500 - 1:2000

Formulation, Preparation, and Storage

Purification

Unpurified

Formulation

Ascites

Format

BSA Free

Preservative

0.03% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: TrkA

The Trk proto-oncogene encodes a 140 kDa, membrane-spanning protein tyrosine kinase that is expressed only in neural tissues. Nerve growth factor (NGF) stimulates phosphorylation of Trk A in neural cell lines and in embryonic dorsal root ganglia. Affinity cross-linking and equilibrium binding experiments with 125I-labeled NGF indicate that Trk A binds NGF specifically in cultured cells with a dissociation constant of 10(-9) molar. The identification of Trk A as an NGF receptor indicates that this protein participates in the primary signal transduction mechanism of NGF (1). Trk A was found to be expressed in the nervous system and phosphorylated in response to NGF (Nerve Growth Factor). Somatic rearrangement(s) of the TRKA gene (also designated NTRK1) are responsible for formation of some oncogenes (2). Trk A is expressed in neural and nonneuronal tissues. Like RET, Trk A is often activated by rearrangements that involve one of at least five other genes in papillary thyroid carcinoma (PTC) (3).

Long Name

Neurotrophic Tyrosine Kinase Receptor A

Alternate Names

NTRK-1, NTRK1

Entrez Gene IDs

4914 (Human)

Gene Symbol

NTRK1

UniProt

P04629

Additional TrkA Products

Product Documents for TrkA Antibody (6B2) - BSA Free

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for TrkA Antibody (6B2) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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TrkA Antibody (6B2) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-47436

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

Format

Concentration

View all TrkA Antibodies »

Product Specifications

Immunogen

Clonality

Host

Isotype

Theoretical MW

Scientific Data Images for TrkA Antibody (6B2) - BSA Free

Western Blot: TrkA Antibody (6B2)BSA Free [NBP1-47436]

Immunocytochemistry/ Immunofluorescence: TrkA Antibody (6B2) - BSA Free [NBP1-47436]

Applications for TrkA Antibody (6B2) - BSA Free

ELISA

Immunocytochemistry/ Immunofluorescence

Western Blot

Formulation, Preparation, and Storage

Purification

Formulation

Format

Preservative

Concentration

Shipping

Stability & Storage

Background: TrkA

Long Name

Alternate Names

Entrez Gene IDs

Gene Symbol

UniProt

Additional TrkA Products

Product Documents for TrkA Antibody (6B2) - BSA Free

Product Datasheets

COA

Certificate of Analysis

SDS

Product Specific Notices for TrkA Antibody (6B2) - BSA Free