Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI2B8

Concentration

1 mg/ml

View all TrkC Antibodies »

Product Summary for TrkC Antibody (OTI2B8)

Immunogen

Full-length protein expressed in 293T cell transfected with human NTRK3 expression vector

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Specificity

This antibody is specific for Homo sapiens neurotrophic tyrosine kinase, receptor, type 3 (NTRK3), transcript variant 3.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

94.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for TrkC Antibody (OTI2B8)

Western Blot: TrkC Antibody (OTI2B8) [NBP1-47900]

Western Blot: TrkC Antibody (OTI2B8) [NBP1-47900]

Western Blot: TrkC Antibody (2B8) [NBP1-47900] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TrkC (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TrkC.
Immunocytochemistry/Immunofluorescence: TrkC Antibody (2B8) [NBP1-47900] Staining of COS7 cells transiently transfected by pCMV6-ENTRY TrkC.
Immunohistochemistry-Paraffin: TrkC Antibody (OTI2B8) [NBP1-47900]

Immunohistochemistry-Paraffin: TrkC Antibody (OTI2B8) [NBP1-47900]

Immunohistochemistry-Paraffin: TrkC Antibody (2B8) [NBP1-47900] - Staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-TrkC mouse monoclonal antibody.
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Applications for TrkC Antibody (OTI2B8)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:50

Immunohistochemistry-Paraffin

1:50

Western Blot

1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: TrkC

This gene encodes a member of the neurotrophic tyrosine receptor kinase (NTRK) family. This kinase is a membrane-bound receptor that, upon neurotrophin binding, phosphorylates itself and members of the MAPK pathway. Signalling through this kinase leads to cell differentiation and may play a role in the development of proprioceptive neurons that sense body position. Mutations in this gene have been associated with medulloblastomas, secretory breast carcinomas and other cancers.

Long Name

Neurotrophic Tyrosine Kinase Receptor C

Alternate Names

NTRK3

Entrez Gene IDs

4916 (Human)

Gene Symbol

NTRK3

UniProt

Q16288

Additional TrkC Products

Product Documents for TrkC Antibody (OTI2B8)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for TrkC Antibody (OTI2B8)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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