TrkC Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-98829

Novus Biologicals, part of Bio-Techne
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NB100-98829
NB100-98829-0.025ml
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

View all TrkC Antibodies »

Product Specifications

Immunogen

A synthetic peptide from the extracellular domain of rat TrkC conjugated to blue carrier protein was used as the antigen. The peptide is homologous in mouse and human.

Localization

Membrane

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for TrkC Antibody

Immunocytochemistry/ Immunofluorescence: TrkC Antibody [NB100-98829]

Immunocytochemistry/ Immunofluorescence: TrkC Antibody [NB100-98829]

Immunocytochemistry/Immunofluorescence: TrkC Antibody [NB100-98829] - Rat DRG at 1 : 500 dilution using Rabbit antibody to extracellular domain of TrkC.
Immunohistochemistry-Paraffin: TrkC Antibody [NB100-98829]

Immunohistochemistry-Paraffin: TrkC Antibody [NB100-98829]

Immunohistochemistry-Paraffin: TrkC Antibody [NB100-98829] - IHC on paraffin sections of rat DRG using Rabbit antibody to TrkC .HIER: 1 mM EDTA, pH 8 for 20 min. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um. Detection was done using HRP polymer following manufacturers instructions. Primary antibody: dilution 10 ug/ml, incubated 30 min at RT (using Autostainer). Sections were counterstained with Harris Hematoxylin.
Immunocytochemistry/ Immunofluorescence: TrkC Antibody [NB100-98829]

Immunocytochemistry/ Immunofluorescence: TrkC Antibody [NB100-98829]

Immunocytochemistry/Immunofluorescence: TrkC Antibody [NB100-98829] - TrkC antibody was tested in neuro2a cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).
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Applications for TrkC Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:1000

Immunohistochemistry

1:1000

Immunohistochemistry-Paraffin

1:1000
Application Notes
This TrkC antibody is useful for Immunohistochemistry-Paraffin and Immunocytochemistry / Immunofluorescence.In ICC/IF, membrane staining was observed in neuro2a cells. In IHC-P, staining was observed in the membrane of mouse brain and spinal cord. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.

Formulation, Preparation, and Storage

Purification

Unpurified

Reconstitution

Reconstitute in 0.1 ml of sterile water. Centrifuge to remove any insoluble material. Glycerol may be added (1:1) for additional stability. Please note the sample size is provided in reconstituted format.

Formulation

Lyophilized from whole antisera

Preservative

No Preservative

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: TrkC

Neurotrophic tyrosine kinase receptor type 3 (NTRK3), commonly known as TRKC, is a member of the protein kinase superfamily, the tyrosine (Tyr) protein kinase family, and more specifically, the insulin receptor subfamily. TRKC is a single pass type I membrane protein that phosphorylates itself (via ligand-mediated auto-phosphorylation) and other proteins in the MAPK pathway upon neurotrophin binding. Mutations to the TRKC gene have been correlated with the development of medulloblastomas, secretory breast carcinomas, and other cancers. TRKC has several transcript variants that encode different isoforms (A= 94.4kDa, B= 68.4kDa, C= 92.8kDa, and D= 93.3kDa), and each isoform has unique signaling properties. Cell differentiation that plays a role in the development of proprioceptive neurons originates via signaling through TRKC. The TRKC protein contains two Ig-like C2-type immunoglobulin-like domains, two leucine-rich repeats (LRR), one leucine rich repeat C-terminal (LRRCT) domain, and one protein kinase domain.

Long Name

Neurotrophic Tyrosine Kinase Receptor C

Alternate Names

NTRK3

Entrez Gene IDs

4916 (Human); 18213 (Mouse); 29613 (Rat)

Gene Symbol

NTRK3

UniProt

Q16288, Q6VNS1, Q03351 (Rat)

Additional TrkC Products

Product Documents for TrkC Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for TrkC Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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