TRMT12 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-76583

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human, Mouse

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1 mg/ml

View all TRMT12 Antibodies »

Product Specifications

Immunogen

Antibody was raised against a 19 amino acid synthetic peptide near the amino terminus of human TYW2. The immunogen is located within the first 50 amino acids of TYW2. Amino Acid Squence: TQRYREYLQRQKLFDTQHR

Specificity

TYW2 antibody is predicted to not cross-react with other TYW protein family members. At least two isoforms of TYW2 are known to exist; this antibody will detect both isoforms

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for TRMT12 Antibody - BSA Free

Western Blot: TRMT12 AntibodyBSA Free [NBP1-76583]

Western Blot: TRMT12 AntibodyBSA Free [NBP1-76583]

Western Blot: TRMT12 Antibody [NBP1-76583] - Western blot analysis of TYW2 in K562 cell lysate with TYW2 antibody at 0.5 ug/mL in (A) the absence and (B) the presence of blocking peptide.

Immunocytochemistry/Immunofluorescence: TRMT12 Antibody [NBP1-76583] -

Immunocytochemistry/Immunofluorescence: TRMT12 Antibody [NBP1-76583] - Immunocytochemistry of TYW2 in K562 cells with TYW2 antibody at 10 ug/mL.

Applications for TRMT12 Antibody - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

10 ug/mL

Western Blot

0.5 ug/ml

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: TRMT12

TYW2, also known as TRM12, is an enzyme that participates in the wybutosine-tRNA (Phe) biosynthesis pathway. Wybutosine (yW) is a hypermodified guanosine at the 3-prime position adjacent to the anticodon of phenylalanine tRNA that stabilizes codon-anticodon interactions during decoding on the ribosome. TYW2 is involved in a multistep enzymatic reaction that stabilizes codon-anticodon base-pairing during the ribosomal decoding process, thereby ensuring correct translation. TYW2 was found to be amplified in 7 of 8 breast cancers cell lines analyzed, and qPCR analysis of 30 breast tumors showed overexpression of TYW2 mRNA of over 2-fold in 26, suggesting that TYW2 may play a role in breast cancer.

Alternate Names

Alpha-amino-alpha-carboxypropyl transferase TYW2, EC 2.1.1, EC 2.1.1.32, EC 2.5.1.-, FLJ20772, homolog of yeast tRNA methyltransferase, TRM12, tRNA methyltranferase 12 homolog, tRNA methyltranferase 12 homolog (S. cerevisiae), tRNA methyltransferase 12 homolog (S. cerevisiae), tRNA-yW synthesizing protein 2, tRNA-yW-synthesizing protein 2, TYW2tRNA wybutosine-synthesizing protein 2 homolog

Gene Symbol

TRMT12

UniProt

Q53H54 (Human)

Additional TRMT12 Products

Product Documents for TRMT12 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for TRMT12 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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