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tropomyosin-3 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-20732

Novus Biologicals, part of Bio-Techne
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NBP2-20732

Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human

Predicted:

Canine (93%), Mouse (99%), Porcine (100%), Rat (99%), Rhesus Macaque (93%). Backed by our 100% Guarantee.

Applications

Knockout Validated, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Full length human Tropomyosin-3 Recombinant protein.

Reactivity Notes

Zebrafish (83%), , Chicken (84%).

Localization

Cytoplasm; cytoskeleton

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

33 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for tropomyosin-3 Antibody

Western Blot: tropomyosin-3 Antibody [NBP2-20732]

Western Blot: tropomyosin-3 Antibody [NBP2-20732]

Western Blot: tropomyosin-3 Antibody [NBP2-20732] - Wild-type (WT) and Tropomyosin 3 knockout (KO) 293T cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Tropomyosin-3 antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Western Blot: tropomyosin-3 Antibody [NBP2-20732]

Western Blot: tropomyosin-3 Antibody [NBP2-20732]

Western Blot: tropomyosin-3 Antibody [NBP2-20732] - Sample (30 ug of whole cell lysate) A: 293T B: A431 12% SDS PAGE gel, diluted at 1:1000.

Applications for tropomyosin-3 Antibody

Application
Recommended Usage

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris (pH 7), 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: tropomyosin-3

This gene encodes a member of the tropomyosin family of actin-binding proteins involved in the contractile system of striated and smooth muscles and the cytoskeleton of non-muscle cells. Tropomyosins are dimers of coiled-coil proteins that polymerize end-to-end along the major groove in most actin filaments. They provide stability to the filaments and regulate access of other actin-binding proteins. In muscle cells, they regulate muscle contraction by controlling the binding of myosin heads to the actin filament. Mutations in this gene result in autosomal dominant nemaline myopathy, and oncogenes formed by chromosomal translocations involving this locus are associated with cancer. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Alternate Names

cytoskeletal tropomyosin TM30, FLJ41118, gamma-tropomyosin, heat-stable cytoskeletal protein 30 kDa, hscp30, hTM5, MGC14582, MGC3261, MGC72094, NEM1, OK/SW-cl.5, TM3, TM30, TM30nm, TM-5, TPMsk3, TRK, tropomyosin 3, tropomyosin alpha-3 chain, tropomyosin gamma, Tropomyosin-3, tropomyosin-5

Gene Symbol

TPM3

Additional tropomyosin-3 Products

Product Documents for tropomyosin-3 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for tropomyosin-3 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.