Troponin C (cardiac) Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-99683

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human, Cynomolgus Monkey

Applications

ELISA, Immunohistochemistry-Paraffin, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Troponin C (cardiac) Antibodies »

Product Summary for Troponin C (cardiac) Antibody

Immunogen

Produced in rabbits immunized with purified, recombinant Human Troponin C (cardiac) (Accession#: NP_003271.1; Met1-Glu161)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for Troponin C (cardiac) Antibody

Immunohistochemistry-Paraffin: Troponin C (cardiac) Antibody [NBP2-99683]

Immunohistochemistry-Paraffin: Troponin C (cardiac) Antibody [NBP2-99683]

Immunohistochemistry-Paraffin: Troponin C (cardiac) Antibody [NBP2-99683] - Immunochemical staining Troponin C (cardiac) in human skeletal muscle with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.
Immunohistochemistry-Paraffin: Troponin C (cardiac) Antibody [NBP2-99683]

Immunohistochemistry-Paraffin: Troponin C (cardiac) Antibody [NBP2-99683]

Immunohistochemistry-Paraffin: Troponin C (cardiac) Antibody [NBP2-99683] - Immunochemical staining Troponin C (cardiac) in cynomolgus heart with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.
Immunoprecipitation: Troponin C (cardiac) Antibody [NBP2-99683]

Immunoprecipitation: Troponin C (cardiac) Antibody [NBP2-99683]

Immunoprecipitation: Troponin C (cardiac) Antibody [NBP2-99683] - Troponin C (cardiac) was immunoprecipitated using: Lane A: 0.5 mg HepG2 Whole Cell Lysate. 4 ul anti-Troponin C (cardiac) rabbit polyclonal antibody and 60 ug of Immunomagnetic beads. Protein A/G. Primary antibody: Anti-Troponin C (cardiac) rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 18 kDa. Observed band size: 16 kDa

Applications for Troponin C (cardiac) Antibody

Application
Recommended Usage

ELISA

1:5000-1:10000

Immunohistochemistry-Paraffin

1:500-1:2000

Immunoprecipitation

5-10 uL/mg of lysate
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen and protein A Affinity-purified

Formulation

0.2 um filtered solution in PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Troponin C (cardiac)

Troponin I is part of a heteromeric complex playing an important role in the regulation of skeletal and cardiac muscle contraction. It consists of three subunits, troponin I (TnI), troponin T (TnT) and troponin C (TnC). Each subunit is responsible for part of troponin complex function. TnI inhibits ATPase activity of acto myosin and TnT and TnI are present in cardiac muscles in different forms than in skeletal muscles. Only one tissue specific isoform of TnI is described for cardiac muscle tissue (cTnI) and this is expressed only in myocardium.

Alternate Names

cardiac troponin C, CMD1Z, CMH13, slow twitch skeletal/cardiac muscle troponin C, TNC, TN-C, TNNC, troponin C type 1 (slow), troponin C, slow, troponin C, slow skeletal and cardiac muscles, troponin C1, slow

Gene Symbol

TNNC1

Additional Troponin C (cardiac) Products

Product Documents for Troponin C (cardiac) Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Troponin C (cardiac) Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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