Troponin I type 2 (fast skeletal) Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-26200
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat, Porcine
Predicted:
Canine (100%). Backed by our 100% Guarantee.
Applications
Peptide ELISA, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Concentration
0.5 mg/ml
Product Specifications
Immunogen
Peptide with sequence KRNRAITARRQHLKS-C corresponding to N-Terminus according to NP_001139313.1.
Specificity
This antibody is expected to recognize both reported isoforms (NP_003273.1; NP_001139313.1). Reported variants represent identical protein: NP_001139301.1, NP_003273.1.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Troponin I type 2 (fast skeletal) Antibody
![Western Blot: Troponin I type 2 (fast skeletal) Antibody [NBP2-26200]](https://resources.bio-techne.com/images/products/Troponin-I-type-2-fast-skeletal-Antibody-Western-Blot-NBP2-26200-img0004.jpg "Western Blot: Troponin I type 2 (fast skeletal) Antibody [NBP2-26200]")
Western Blot: Troponin I type 2 (fast skeletal) Antibody [NBP2-26200]
Western Blot: Troponin I type 2 (fast skeletal) Antibody [NBP2-26200] - Porcine muscle RIPA extract, 15 ug per well. Primary antibody 1:1000. Blocker 5% rabbit serum. Samples: 1) longissimus dorsi (fast twitch); 2) flexor digitorum (slow twitch); 3) vastus lateralis (fast twitch); diaphragm (mixed); 5) cardiac. This image was submitted via customer review.
Western Blot: Troponin I type 2 (fast skeletal) Antibody [NBP2-26200] -
Western Blot: Troponin I type 2 (fast skeletal) Antibody [NBP2-26200] - (0.03ug/ml) staining of Human (A), Mouse (B) and Rat (C) Skeletal Muscle lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.Applications for Troponin I type 2 (fast skeletal) Antibody
Application
Recommended Usage
Peptide ELISA
Detection limit 1:128000
Western Blot
0.03 - 0.1 ug/ml
Application Notes
WB: Approx. 25 kDa band observed in human, mouse and rat skeletal muscle lysates (calculated MW of 21.3 kDa band according to NP_003273.1).
Reviewed Applications
Read 1 review rated 5 using NBP2-26200 in the following applications:
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Troponin I type 2 (fast skeletal)
Alternate Names
skeletal, fast, troponin I type 2 (skeletal, fast), troponin I, fast skeletal muscle, Troponin I, fast-twitch isoform, troponin I, fast-twitch skeletal muscle isoform
Gene Symbol
TNNI2
Additional Troponin I type 2 (fast skeletal) Products
Product Documents for Troponin I type 2 (fast skeletal) Antibody
Product Specific Notices for Troponin I type 2 (fast skeletal) Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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