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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse

Applications

Validated:

Flow Cytometry, Immunoaffinity Purification, Immunoassay, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Sandwich ELISA, Western Blot

Cited:

Flow Cytometry, IF/IHC, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 19C7cc

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Hybridoma clone has been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with free human cTnI.

Epitope

a.a.r. 41-49

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Specificity

Troponin I Type 3 (cardiac). No cross-reacticity with skeletal muscle TnI.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Troponin I Type 3 (cardiac) Antibody (19C7cc)

Immunohistochemistry-Paraffin: Troponin I Type 3 (cardiac) Antibody (19C7cc) [NB110-2546]

Immunohistochemistry-Paraffin: Troponin I Type 3 (cardiac) Antibody (19C7cc) [NB110-2546]

Immunohistochemistry-Paraffin: Troponin I Type 3 (cardiac) Antibody (19C7cc) [NB110-2546] - Mouse cardiac tissue stained with Troponin I Type 3 antibody. IHC-P image submitted by a verifed customer review.
Flow Cytometry: Troponin I Type 3 (cardiac) Antibody (19C7cc) [NB110-2546]

Flow Cytometry: Troponin I Type 3 (cardiac) Antibody (19C7cc) [NB110-2546]

Flow Cytometry: Troponin I Type 3 (cardiac) Antibody (19C7) [NB110-2546] - FACS analysis of Troponin I Type 3 in differentiated ESC cultures using anti-Troponin I Type 3 (cardiac) antibody. Image from verified customer review.

Applications for Troponin I Type 3 (cardiac) Antibody (19C7cc)

Application
Recommended Usage

Flow Cytometry

1:10-1:1000

Immunohistochemistry

1:10-1:500

Immunoprecipitation

1:10-1:500

Sandwich ELISA

1:100-1:2000

Western Blot

1:100-1:2000
Application Notes
Recommended pairs for sandwich immunoassay (capture-detection): NB110-2546 - NB100-73082, NB110-2546 - NB100-73087, NB100-73085 - NB110-2546, or NB110-8453 - NB110-2546.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 2 reviews rated 5 using NB110-2546 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.4)

Preservative

0.09% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Troponin I/TNNI3

Equally reactive with free cardiac troponin I (cTnI) and cTnI forming complexes with other troponin components. (In the presence of 5 mM EDTA). Antibodies are not affected by heparin, phosphorylation, oxidation and troponin complex formation. No cross-reaction with skeletal muscle troponin I.

Long Name

Troponin I3, Cardiac Type

Alternate Names

CMD2A, CTnI, RCM1, TNNC1, TNNI3,

Entrez Gene IDs

7137 (Human); 21955 (Mouse); 171409 (Rat)

Gene Symbol

TNNI3

UniProt

Additional Troponin I/TNNI3 Products

Product Documents for Troponin I Type 3 (cardiac) Antibody (19C7cc)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Troponin I Type 3 (cardiac) Antibody (19C7cc)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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