Troponin I Type 3 (cardiac) Antibody (1E10) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-35337

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human, Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # 1E10

Format

BSA Free

Concentration

1.0 mg/ml

View all Troponin I/TNNI3 Antibodies »

Product Summary for Troponin I Type 3 (cardiac) Antibody (1E10) - BSA Free

Immunogen

Partial recominant human Troponin 1 (between amino acids 1-200) [UniProt P19429]

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Scientific Data Images for Troponin I Type 3 (cardiac) Antibody (1E10) - BSA Free

Western Blot: Troponin I Type 3 (cardiac) Antibody (1E10)BSA Free [NBP2-35337]

Western Blot: Troponin I Type 3 (cardiac) Antibody (1E10)BSA Free [NBP2-35337]

Western Blot: Troponin I Type 3 (cardiac) Antibody (1E10) [NBP2-35337] - Western blot analysis of clone 1E10 in human heart at 0.5 and 1.0 ug/ml.
Immunohistochemistry-Paraffin: Troponin I Type 3 (cardiac) Antibody (1E10) - BSA Free [NBP2-35337]

Immunohistochemistry-Paraffin: Troponin I Type 3 (cardiac) Antibody (1E10) - BSA Free [NBP2-35337]

Immunohistochemistry-Paraffin: Troponin I Type 3 (cardiac) Antibody (1E10) [NBP2-35337] - IHC analysis of formalin-fixed paraffin-embedded tissue section of human heart using Troponin I antibody (clone 1E10) at 5 ug/ml concentration. The antibody specifically detected the target protein in cardiac muscle fibers in the tested sections, whereas, the nuclei as well as the intercellular collagen network did not develop any immunostaining.
Immunohistochemistry-Paraffin: Troponin I Type 3 (cardiac) Antibody (1E10) - BSA Free [NBP2-35337]

Immunohistochemistry-Paraffin: Troponin I Type 3 (cardiac) Antibody (1E10) - BSA Free [NBP2-35337]

Immunohistochemistry-Paraffin: Troponin I Type 3 (cardiac) Antibody (1E10) [NBP2-35337] - IHC-P testing of a formalin fixed tissue section of human liver with 5ug/ml concentration of Troponin I Type 3 (cardiac) antibody (clone 1E10) showing no staining - Negative Control Tissue

Applications for Troponin I Type 3 (cardiac) Antibody (1E10) - BSA Free

Application
Recommended Usage

Immunohistochemistry

5 ug/ml

Immunohistochemistry-Paraffin

5 ug/ml

Western Blot

0.5 ug/ml
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Troponin I/TNNI3

Troponin I is part of a heteromeric complex playing an important role in the regulation of skeletal and cardiac muscle contraction. It consists of three subunits, troponin I (TnI), troponin T (TnT) and troponin C (TnC). Each subunit is responsible for part of troponin complex function. TnI inhibits ATPase activity of acto myosin and TnT and TnI are present in cardiac muscles in different forms than in skeletal muscles. Only one tissue specific isoform of TnI is described for cardiac muscle tissue (cTnI) and this is expressed only in myocardium. This antibody reacts with subunit I of the troponin complex in human myocardium, where it is the only troponin I isotype present. It does not cross-react with troponin I of skeletal muscle cells.

Long Name

Troponin I3, Cardiac Type

Alternate Names

CMD2A, CTnI, RCM1, TNNC1, TNNI3,

Entrez Gene IDs

7137 (Human)

Gene Symbol

TNNI3

UniProt

P19429

Additional Troponin I/TNNI3 Products

Product Documents for Troponin I Type 3 (cardiac) Antibody (1E10) - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Troponin I Type 3 (cardiac) Antibody (1E10) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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