Key Product Details

Species Reactivity

Validated:

Human, Porcine, Bovine (Negative), Canine (Negative), Feline (Negative), Fish (Negative), Goat (Negative), Mouse (Negative), Rabbit (Negative), Rat (Negative)

Applications

ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 22B11

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Troponin I/TNNI3 Antibodies »

Product Summary for Troponin I Type 3 (cardiac) Antibody (22B11)

Immunogen

Hybridoma clone has been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with cTnI.

Epitope

a.a.r. 20-24

Reactivity Notes

Cross-reacts with Human and Pig. Does not cross-react with Mouse, Rat, Cow, Cat, Dog, Fish, Goat or Rabbit. Not yet tested in other species.

Specificity

This detects the unphosphorylated form of cardiac troponin I.No cross-reaction with mono or biphosphorylated troponin I.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Troponin I Type 3 (cardiac) Antibody (22B11)

Western Blot: Troponin I Type 3 (cardiac) Antibody (22B11) [NB120-8290]

Western Blot: Troponin I Type 3 (cardiac) Antibody (22B11) [NB120-8290]

Western Blot: Troponin I (cardiac) Antibody (22B11)[NB120-8290]-Momoclonal antibodies 22B11(A), 10B11(B), and 8E10(C) recognizing dephospho- and phospho- forms of human cardiac Tnl.

Applications for Troponin I Type 3 (cardiac) Antibody (22B11)

Application
Recommended Usage

ELISA

1:100-1:2000

Western Blot

1:100-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.4)

Preservative

0.09% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Troponin I/TNNI3

Troponin I is part of a heteromeric complex playing an important role in the regulation of skeletal and cardiac muscle contraction. It consists of three subunits, troponin I (TnI), troponin T (TnT) and troponin C (TnC). Each subunit is responsible for part of troponin complex function. TnI inhibits ATPase activity of acto myosin and TnT and TnI are present in cardiac muscles in different forms than in skeletal muscles. Only one tissue specific isoform of TnI is described for cardiac muscle tissue (cTnI) and this is expressed only in myocardium. This antibody reacts with subunit I of the troponin complex in human myocardium, where it is the only troponin I isotype present. It does not cross-react with troponin I of skeletal muscle cells.

Long Name

Troponin I3, Cardiac Type

Alternate Names

CMD2A, CTnI, RCM1, TNNC1, TNNI3,

Entrez Gene IDs

7137 (Human); 21955 (Mouse); 171409 (Rat)

Gene Symbol

TNNI3

Additional Troponin I/TNNI3 Products

Product Documents for Troponin I Type 3 (cardiac) Antibody (22B11)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Troponin I Type 3 (cardiac) Antibody (22B11)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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