Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunoaffinity Purification, Immunoassay, Immunohistochemistry, Immunoprecipitation, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 625

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Troponin I/TNNI3 Antibodies »

Product Specifications

Immunogen

Hybridoma clone has been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with native human cTn complex.

Epitope

a.a.r. 169-178

Specificity

No cross-reaction with skeletal muscle TnI

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Applications for Troponin I Type 3 (cardiac) Antibody (625)

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunoaffinity Purification

Optimal dilutions of this antibody should be experimentally determined.

Immunoassay

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunoprecipitation

Optimal dilutions of this antibody should be experimentally determined.

Sandwich ELISA

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Recommended pairs for quantitative sandwich immunoassay (capture-detection): NB100-73085 - NB110-2546
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.4)

Preservative

0.09% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Troponin I/TNNI3

Troponin I is part of a heteromeric complex playing an important role in the regulation of skeletal and cardiac muscle contraction. It consists of three subunits, troponin I (TnI), troponin T (TnT) and troponin C (TnC). Each subunit is responsible for part of troponin complex function. TnI inhibits ATPase activity of acto myosin and TnT and TnI are present in cardiac muscles in different forms than in skeletal muscles. Only one tissue specific isoform of TnI is described for cardiac muscle tissue (cTnI) and this is expressed only in myocardium. This antibody reacts with subunit I of the troponin complex in human myocardium, where it is the only troponin I isotype present. It does not cross-react with troponin I of skeletal muscle cells.

Long Name

Troponin I3, Cardiac Type

Alternate Names

CMD2A, CTnI, RCM1, TNNC1, TNNI3,

Entrez Gene IDs

7137 (Human)

Gene Symbol

TNNI3

Additional Troponin I/TNNI3 Products

Product Documents for Troponin I Type 3 (cardiac) Antibody (625)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Troponin I Type 3 (cardiac) Antibody (625)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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