Troponin I Type 3 (cardiac) [p Ser23, p Ser24] Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-29525

Novus Biologicals, part of Bio-Techne
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NBP2-29525
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Predicted:

Human (100%), Primate (100%). Backed by our 100% Guarantee.

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Troponin I/TNNI3 Antibodies »

Product Summary for Troponin I Type 3 (cardiac) [p Ser23, p Ser24] Antibody

Immunogen

Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser23/24 conjugated to KLH. Accession # P48787

Modification

p Ser23, p Ser24

Specificity

Specific for endogenous levels of the ~25 kDa Troponin I Type 3 (cardiac) protein phosphorylated at Ser23/24. Immunolabeling is blocked by preadsorption with the phosphopeptide used as antigen, but not by the corresponding non-phosphopeptide.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

25 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Troponin I Type 3 (cardiac) [p Ser23, p Ser24] Antibody

Western Blot: Troponin I Type 3 (cardiac) [p Ser23, p Ser24] Antibody [NBP2-29525]

Western Blot: Troponin I Type 3 (cardiac) [p Ser23, p Ser24] Antibody [NBP2-29525]

Western Blot: Troponin I Type 3 (cardiac) [p Ser23, p Ser24] Antibody [NBP2-29525] - Western blot of mouse heart homogenate showing specific immunolabeling of the ~25k cTnI protein phosphorylated at Ser23/24 (control). Immunolabeling is blocked by preadsorption with the phospho-peptide used as antigen (Peptide), but not by the corresponding dephospho-peptide (not shown).

Applications for Troponin I Type 3 (cardiac) [p Ser23, p Ser24] Antibody

Application
Recommended Usage

Western Blot

1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

10mM HEPES (pH 7.5), 0.15M NaCl, 0.1 mg/ml BSA and 50% Glycerol

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Troponin I/TNNI3

Troponin I (cTnI) is 1 of 3 subunits, along with troponin C (TnC) and troponin T (TnT) of troponin complex found in cardiac muscle. cTnI binds to actin in thin myofilaments to hold the troponin-tropomyosin complex in place, and when cTnI is phosphorylated by protein kinase C and protein kinase A at Ser23/24 it causes regulation of Ca2+- stimulated ATPase (Noland et al, 1995). Evidence suggests that phosphorylation of both serines 23 and 24 is required for the reduction in Ca2+-sensitivity and beneficial for relaxation of the heart (Kooij et al, 2010). Ser23/24 phosphorylation is important for enhanced relaxation in response to prolonged activation of protein kinase C by endothelin in intact myocytes, while Thr144 plays an important role in the acute acceleration of relaxation (Westfall et al, 2005).

Long Name

Troponin I3, Cardiac Type

Alternate Names

CMD2A, CTnI, RCM1, TNNC1, TNNI3,

Entrez Gene IDs

21954 (Mouse)

Gene Symbol

TNNI3

UniProt

P48787 (Mouse)

Additional Troponin I/TNNI3 Products

Product Documents for Troponin I Type 3 (cardiac) [p Ser23, p Ser24] Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Troponin I Type 3 (cardiac) [p Ser23, p Ser24] Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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