Troponin I Type 3 (cardiac) [p Ser150] Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-29526

Novus Biologicals, part of Bio-Techne
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NBP2-29526
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Mouse

Predicted:

Human (100%), Primate (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Troponin I/TNNI3 Antibodies »

Product Specifications

Immunogen

Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser150 conjugated to KLH. Accession # P48787

Modification

p Ser150

Specificity

Specific for endogenous levels of the ~25 kDa Troponin I Type 3 (cardiac) protein phosphorylated at Ser150. Immunolabeling is greatly decreased with lambda-phosphatase treatment.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

25 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Troponin I Type 3 (cardiac) [p Ser150] Antibody

Western Blot: Troponin I Type 3 (cardiac) [p Ser150] Antibody [NBP2-29526]

Western Blot: Troponin I Type 3 (cardiac) [p Ser150] Antibody [NBP2-29526]

Western Blot: Troponin I Type 3 (cardiac) [p Ser150] Antibody [NBP2-29526] - Western blot of mouse heart lysate showing specific immunolabeling of ~25k cTnI protein phosphorylated at Ser150 (control). Phosphospecificity is shown in the second lane. The blot is identical to the control except that the lysate was incubated in lambda-Ptase (1400 units for 30 min). The immunolabeling is greatly decreased by treatment with lambda-Ptase.
Immunofluorescence: Troponin I Type 3 (cardiac) [p Ser150] Antibody [NBP2-29526]

Immunofluorescence: Troponin I Type 3 (cardiac) [p Ser150] Antibody [NBP2-29526]

Immunofluorescence: Troponin I Type 3 (cardiac) [p Ser150] Antibody [NBP2-29526] - Fixed adult mouse cardiomyocyte were stained with anti-Pim-1 and anti-cTnI antibodies and then processed for fluorescentstaining analysis. From Zhu et al.: Phosphorylation of cTnI by Pim1 Kinase, Cell Physiol Biochem 2018;45:2174-2186.
Immunoprecipitation: Troponin I Type 3 (cardiac) [p Ser150] Antibody [NBP2-29526]

Immunoprecipitation: Troponin I Type 3 (cardiac) [p Ser150] Antibody [NBP2-29526]

Immunoprecipitation: Troponin I Type 3 (cardiac) [p Ser150] Antibody [NBP2-29526] - Adult mouse heart tissue samples were extracted and immunoprecipitated with either anti-Pim-1 antibody or control IgG and then analyzed by Westernblot. From Zhu et al.: Phosphorylation of cTnI by Pim1 Kinase, Cell Physiol Biochem 2018;45:2174-2186.

Applications for Troponin I Type 3 (cardiac) [p Ser150] Antibody

Application
Recommended Usage

Western Blot

1:1000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

10mM HEPES (pH 7.5), 0.15M NaCl, 0.1 mg/ml BSA and 50% Glycerol

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Troponin I/TNNI3

Troponin I (TnI) is 1 of 3 subunits, along with troponin C (TnC) and Troponin T (TnT) of troponin complex found in cardiac (cTnI) and fast skeletal (fsTnI) muscle. cTnI is phosphorylated by protein kinase C and protein kinase A at Ser23/24 (Noland et al, 1995) and is phosphorylated by AMPK at Ser23 and Ser150 (Solis et al, 2011). Evidence suggests that AMPK, a critical regulator of cardiac energetics, prefers phosphorylating Ser150 over Ser23, and may play a role in regulating energy consumption through altering the phosphorylation status of cTnI (Solis et al., 2011).

Long Name

Troponin I3, Cardiac Type

Alternate Names

CMD2A, CTnI, RCM1, TNNC1, TNNI3,

Entrez Gene IDs

21954 (Mouse)

Gene Symbol

TNNI3

UniProt

P48787 (Mouse)

Additional Troponin I/TNNI3 Products

Product Documents for Troponin I Type 3 (cardiac) [p Ser150] Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Troponin I Type 3 (cardiac) [p Ser150] Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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