Troponin I Type 3 (cardiac) Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-33463

Novus Biologicals, part of Bio-Techne
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NBP2-33463
NBP2-33463-25ul
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Predicted:

Mouse (90%), Rat (90%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Troponin I/TNNI3 Antibodies »

Product Summary for Troponin I Type 3 (cardiac) Antibody

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: PIRRRSSNYRAYATEPHAKKKSKISASRKLQLKTLLLQIAKQELEREAEERRGEKGRALSTRCQPLELAGLGFAELQ

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Troponin I Type 3 (cardiac) Antibody

Immunocytochemistry/ Immunofluorescence: Troponin I Type 3 (cardiac) Antibody [NBP2-33463]

Immunocytochemistry/ Immunofluorescence: Troponin I Type 3 (cardiac) Antibody [NBP2-33463]

Immunocytochemistry/Immunofluorescence: Troponin I Type 3 (cardiac) Antibody [NBP2-33463] - Staining of human cell line HEK 293 shows localization to cytosol & vesicles.
Immunohistochemistry-Paraffin: Troponin I Type 3 (cardiac) Antibody [NBP2-33463]

Immunohistochemistry-Paraffin: Troponin I Type 3 (cardiac) Antibody [NBP2-33463]

Immunohistochemistry-Paraffin: Troponin I Type 3 (cardiac) Antibody [NBP2-33463] - Staining of human prostate shows low expression as expected.
Immunohistochemistry: Troponin I Type 3 (cardiac) Antibody [NBP2-33463]

Immunohistochemistry: Troponin I Type 3 (cardiac) Antibody [NBP2-33463]

Immunohistochemistry: Troponin I Type 3 (cardiac) Antibody [NBP2-33463] - Staining of human heart muscle shows moderate cytoplasmic positivity in myocytes.
View 1 more images

Applications for Troponin I Type 3 (cardiac) Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:1000 - 1:2500

Immunohistochemistry-Paraffin

1:1000 - 1:2500
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Troponin I/TNNI3

Equally reactive with free cardiac troponin I (cTnI) and cTnI forming complexes with other troponin components. (In the presence of 5 mM EDTA). Antibodies are not affected by heparin, phosphorylation, oxidation and troponin complex formation. No cross-reaction with skeletal muscle troponin I.

Long Name

Troponin I3, Cardiac Type

Alternate Names

CMD2A, CTnI, RCM1, TNNC1, TNNI3,

Gene Symbol

TNNI3

UniProt

P19429

Additional Troponin I/TNNI3 Products

Product Documents for Troponin I Type 3 (cardiac) Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Troponin I Type 3 (cardiac) Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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