Troponin T Type 2 (cardiac) Antibody (1C11cc)
Novus Biologicals, part of Bio-Techne | Catalog # NB200-412
Key Product Details
Species Reactivity
Human, Mouse, Rat, Canine
Applications
Immunoassay, Sandwich ELISA, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 1C11cc
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Hybridoma clone has been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with free human cTnT.
Epitope
a.a.r. 171-190
Reactivity Notes
Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.
Localization
Located on the thin filament of striated muscles.
Specificity
No cross-reaction with skeletal TnT
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Troponin T Type 2 (cardiac) Antibody (1C11cc)
Sandwich ELISA: Troponin T Type 2 (cardiac) Antibody (1C11) [NB200-412] - Calibrating curves for sandwich cTnT fluoroimmunoassay with different animal TnTs as antigen: human, canine, rat, mouse Clone 1C11 for capture (1 ug/well) and Clone b 7E7 for detection (200 ng/well). Assay time: 30 min. Room temperature. Hybridoma cell line producing Clone 7E7 was raised after hybridization of myeloma cell line Sp2/0 with splenocytes of mice immunized with human tertiary Tn complex. Thus Clone 7E7 demonstrates slight cross-reaction with cTnI (about 5% based on ELISA data).
Applications for Troponin T Type 2 (cardiac) Antibody (1C11cc)
Application
Recommended Usage
Sandwich ELISA
1:100-1:2000
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS (pH 7.4)
Preservative
0.09% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: Troponin T Type 2 (cardiac)
Alternate Names
cardiac, CMD1D, CMPD2, dilated 1D (autosomal dominant), hypertrophic 2, MGC3889, RCM3, TnTc, troponin T type 2 (cardiac), troponin T, cardiac muscle
Gene Symbol
TNNT2
Additional Troponin T Type 2 (cardiac) Products
Product Documents for Troponin T Type 2 (cardiac) Antibody (1C11cc)
Product Specific Notices for Troponin T Type 2 (cardiac) Antibody (1C11cc)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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