TRPA1 Antibody

Western Blot: TRPA1 Antibody [NB100-91319]

Western Blot: TRPA1 Antibody [NB100-91319] - Tissue lysates. Blocking: 1% LFDM for 30 min at RT; primary antibody: dilution 1:6000 incubated overnight at 4C.

Western Blot: TRPA1 Antibody [NB100-91319] -

Western Blot: TRPA1 Antibody [NB100-91319] - TRPA1 inhibitor HC030031 significantly ameliorated the oxidative stress & apoptosis levels of Pg.LPS-induced PDLCs. (a) P.g.LPS increased intracellular calcium ion level in a dose-dependent manner (n = 3). (b) Lower HC030031 concentration significantly reversed the increase of intracellular calcium ions, & 10 μM HC030031 was used in the subsequent experiment (n = 3). (c) Flow cytometry analysis of the control group (PDLCs only, C), L group (PDLCs treated by LPS, L), & LH group (PDLCs treated by 10 μM HC030031 & LPS, LH) (n = 4). (d, e) Western blot analysis & semiquantitative statistical analysis of oxidative stress, apoptosis, & TRPA1 proteins in in C, L, & LH groups (n = 3). (f) EM images showing endoplasmic reticulum (yellow arrows) & mitochondrial morphology (red arrows) of PDLCs in Ctr, LPS, & LH groups (white stars represent cell nuclei) (n = 4). (g) Quantification of endoplasmic reticulum size, mitochondrial size, mitochondrial number per cell (n = 4), & mitochondrial crista density was analyzed (>100 mitochondria). Data analysis was performed by using one-way ANOVA (∗P < 0.05, ∗∗P < 0.01, & ∗∗∗P < 0.001). Data are presented as the mean ± SEM. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/35720191), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: TRPA1 Antibody [NB100-91319] -

Western Blot: TRPA1 Antibody [NB100-91319] - Periodontitis-derived periodontal ligament cells & tissues were at high levels of oxidative stress & apoptosis. (a) The related gene expressions of oxidative stress, apoptosis, & some of the TRP families in healthy & periodontitis-derived periodontal ligament cells (PDLCs & P-PDLCs) (n = 3). (b, c) Western blot & semiquantitative statistical analysis of oxidative stress, apoptosis, & TRPA1 in PDLCs & P-PDLCs. (n = 3). d, Flow cytometry analysis of PDLCs & P-PDLCs (n = 4). (e, f) H&E staining (white star represent immune cell infiltration), immunohistochemistry & immunofluorescence staining, & semiquantitative statistical analysis of periodontitis & healthy derived periodontal ligament tissues (PDLTs & P-PDLTs) (n = 3). Data analysis was performed by using Student's t-test (∗P < 0.05, ∗∗P < 0.01, & ∗∗∗P < 0.001). The data are presented as the mean ± SEM. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/35720191), licensed under a CC-BY license. Not internally tested by Novus Biologicals.