TRPC1 Antibody (JE35-25)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-33060
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Applications
Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # JE35-25
Concentration
1 mg/ml
Product Summary for TRPC1 Antibody (JE35-25)
Immunogen
Recombinant protein within Human TRPC1 aa 408-507 / 793. (Uniprot: P48995)
Localization
Membrane.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
91 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for TRPC1 Antibody (JE35-25)
Western Blot: TRPC1 Antibody (JE35-25) [NBP3-33060]
Western Blot: TRPC1 Antibody (JE35-25) [NBP3-33060] - Western blot analysis of TRPC1 on different lysates with Rabbit anti-TRPC1 antibody (NBP3-33060) at 1/1,000 dilution.Lane 1: SH-SY5Y cell lysate 15 ug/Lane)
Lane 2: HepG2 cell lysate 15 ug/Lane)
Lane 3: A549 cell lysate 15 ug/Lane)
Lane 4: MCF7 cell lysate 15 ug/Lane)
Lane 5: SK-OV-3 cell lysate 15 ug/Lane)
Lane 6: NIH/3T3 cell lysate 15 ug/Lane)
Lane 7: PC-12 cell lysate 15 ug/Lane)
Lane 8: Human brain tissue lysate (30 ug/Lane)
Lane 9: Mosue brain tissue lysate (30 ug/Lane)
Lane 10: Mosue testis tissue lysate (30 ug/Lane)
Lane 11: Rat testis tissue lysate (30 ug/Lane)
Predicted band size: 91 kDa
Observed band size: 120 kDa
Exposure time: 3 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33060) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.
Applications for TRPC1 Antibody (JE35-25)
Application
Recommended Usage
Western Blot
1:1000-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
1*TBS (pH7.4), 0.05% BSA and 40% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: TRPC1
Long Name
Short transient receptor potential channel 1
Alternate Names
TRP-1, TRP1
Gene Symbol
TRPC1
Additional TRPC1 Products
Product Documents for TRPC1 Antibody (JE35-25)
Product Specific Notices for TRPC1 Antibody (JE35-25)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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