Key Product Details

Validated by

Biological Validation

Species Reactivity

Human

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all TRPC3 Antibodies »

Product Specifications

Immunogen

The immunogen this antibody was made to, maps to a region between residue 275 to 325 of human Transient Receptor Potential Cation Channel, Subfamily C, Member 3 using the numbering given in entry Q13507.3 (GeneID 7222).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for TRPC3 Antibody

Western Blot: TRPC3 Antibody [NBP2-32258]

Western Blot: TRPC3 Antibody [NBP2-32258]

Western Blot: TRPC3 Antibody [NBP2-32258] - Samples: Whole cell lysate (50 ug) prepared using NETN or RIPA buffer from HeLa, 293T, and Jurkat cells. Antibodies: Affinity purified rabbit anti-TRPC3 antibody NBP2-32258 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Immunoprecipitation: TRPC3 Antibody [NBP2-32258]

Immunoprecipitation: TRPC3 Antibody [NBP2-32258]

Immunoprecipitation: TRPC3 Antibody [NBP2-32258] - Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) prepared using NETN buffer from 293T cells. Antibodies: Affinity purified rabbit anti-TRPC3 antibody NBP2-32258 used for IP at 6 ug per reaction. TRPC3 was also immunoprecipitated by rabbit anti-TRPC3 antibody BL16089. For blotting immunoprecipitated TRPC3, NBP2-32258 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Applications for TRPC3 Antibody

Application
Recommended Usage

Immunoprecipitation

2 - 10 ug/mg lysate

Western Blot

1:2000 - 1:10000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: TRPC3

The transient receptor potential canonical (TRPC) group of channels, including TPRC3, is a family of receptor-activated non-selective calcium permeant cation channels. In vitro TRPC proteins can form hetero- or homo-oligomeric channels. Endogenous TRPC1, TRPC3 and TRPC7 participate in forming heteromeric store-operated channels, while TRPC3 and TRPC7 can also participate in forming heteromeric receptor-operated channels. TRPC3 plays important roles in neuronal differentiation and immune cell maturation by mediating the cationic current in response to phospholipase C activation, Ca(2+) depletion, and diacylglycerol stimulation. TRPC3 channels are highly expressed in pontine neurons and mediate a nonselective cation current in response to the neurotrophin receptor, TrkB.

Long Name

Short transient receptor potential channel 3

Alternate Names

hTrp-3, hTrp3, TRP-3, TRP3

Gene Symbol

TRPC3

UniProt

Q13507.3 (Human)

Additional TRPC3 Products

Product Documents for TRPC3 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for TRPC3 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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