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TRPC6 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-31008

Novus Biologicals, part of Bio-Techne
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NBP1-31008

Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human, Mouse

Predicted:

Bovine (92%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human TRPC6. The exact sequence is proprietary.

Localization

Membrane, Multi-pass membrane protein

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

106 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for TRPC6 Antibody

Western Blot: TRPC6 Antibody [NBP1-31008]

Western Blot: TRPC6 Antibody [NBP1-31008]

Western Blot: TRPC6 Antibody [NBP1-31008] - A549 whole cell and membrane extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with TRPC6 antibody diluted at 1:3000.
Immunohistochemistry-Paraffin: TRPC6 Antibody [NBP1-31008]

Immunohistochemistry-Paraffin: TRPC6 Antibody [NBP1-31008]

Immunohistochemistry-Paraffin: TRPC6 Antibody [NBP1-31008] - TRPC6 antibody [C3], C-term detects TRPC6 protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse cerebellum.Green: TRPC6 stained by TRPC6 antibody [C3], C-term (NBP1-31008).Red: NF-H, a neuron marker, stained by NF-H antibody [GT114].Blue: Fluoroshield with DAPI.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Western Blot: TRPC6 Antibody [NBP1-31008]

Western Blot: TRPC6 Antibody [NBP1-31008]

Western Blot: TRPC6 Antibody [NBP1-31008] - Sample(30 ug of whole cell lysate)A:HeLa S3 7.5% SDS PAGE, antibody diluted at 1:3000.

Applications for TRPC6 Antibody

Application
Recommended Usage

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS (pH 7), 40% Glycerol

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: TRPC6

FUNCTION: Thought to form a receptor-activated non-selective calcium permanent cation channel. Probably is operated by a phosphatidylinositol second messenger system activated by receptor tyrosine kinases or G-protein coupled receptors. Activated by diacylglycerol (DAG) in a membrane-delimited fashion, independently of protein kinase C. Seems not to be activated by intracellular calcium store depletion. SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.

Long Name

Short transient receptor potential channel 6

Alternate Names

FSGS2, TRP-6, TRP6

Gene Symbol

TRPC6

Additional TRPC6 Products

Product Documents for TRPC6 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for TRPC6 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.