TRPM2 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NB110-81601

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Mouse, Rat

Cited:

Mouse, Rat

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot

Cited:

IF/IHC, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1.0 mg/ml

View all TRPM2 Antibodies »

Product Specifications

Immunogen

Synthetic peptide made to a C-terminal portion of the rat TRPM2 protein (within residues 1430-1508). [Swiss-Prot# Q5G856]

Localization

Cell membrane.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

181 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for TRPM2 Antibody - BSA Free

Western Blot: TRPM2 AntibodyBSA Free [NB110-81601]

Western Blot: TRPM2 AntibodyBSA Free [NB110-81601]

Western Blot: TRPM2 Antibody [NB110-81601] - Lane 1: CHO cells untransfected. Lane 2: CHO cell transfected with rat TRPM2 Lane 3: Mouse cortical neurons (cultured) Photo courtesy of Dr. Paco Herson, Oregon Health & Science University.
Immunocytochemistry/ Immunofluorescence: TRPM2 Antibody - BSA Free [NB110-81601]

Immunocytochemistry/ Immunofluorescence: TRPM2 Antibody - BSA Free [NB110-81601]

Immunocytochemistry/Immunofluorescence: TRPM2 Antibody [NB110-81601] - Neuro2a cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton X-100. The cells were incubated with anti-TRPM2 at 5 ug/mL overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
Immunocytochemistry/ Immunofluorescence: TRPM2 Antibody - BSA Free [NB110-81601]

Immunocytochemistry/ Immunofluorescence: TRPM2 Antibody - BSA Free [NB110-81601]

Immunocytochemistry/Immunofluorescence: TRPM2 Antibody [NB110-81601] - Staining of rat hippocampus using NB110-81601. TRPM2 is stained red, GFAP is stained green and nuclei staining is blue. Photo courtesy of Dr. Ji-Zhong Bai, The University of Auckland, New Zealand.
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Applications for TRPM2 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:10 - 1:500

Immunohistochemistry

1:200

Immunohistochemistry-Paraffin

1:200

Western Blot

1:500 - 1:1000
Application Notes
In Western blot a band is seen at ~181 kDa. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: TRPM2

TRP7 (transient receptor protein 7 or TRPC7) is the seventh identified member of mammalian TRPC channel family which includes nonselective cation channels that mediate changes in plasma membrane cation permeability induced by stimulation of PLC (phospholipase C, the cellular integrator that receives input from GPCRs/ RTK). Major cellular function of TRPC channels is to regulate Ca2+ influx across plasma membrane induced by stimulation of these receptors by hormones, neurotransmitters, and growth factors. Localized in the cell membrane and nuclear envelope as multi-pass membrane protein, TRPC7 interacts with with MX1, RNF24, PRKG1 etc. and PRKG1 mediated TRPC7 phosphorylation at Thr-15 site negatively regulates TRPC7's activity. TRP7 has been suggested to form a receptor-activated non-selective calcium permeant cation channel and is operated by a phosphatidylinositol second messenger system activated by RTKs or GPCRs. TRP7 is activated by DAG (diacylglycerol) and also upon intracellular calcium store depletion.

Alternate Names

EC 3.6.1.13, EREG1MGC133383, Estrogen-responsive element-associated gene 1 protein, KNP3LTrpC-2, Long transient receptor potential channel 2, LTrpC2, LTRPC2TRPC7transient receptor potential cation channel subfamily M member 2, NUDT9H, NUDT9L1, transient receptor potential cation channel, subfamily M, member 2, Transient receptor potential channel 7, TrpC7

Gene Symbol

TRPM2

Additional TRPM2 Products

Product Documents for TRPM2 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for TRPM2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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