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TRPM2 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NB110-82364

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1.5 mg/ml

Product Specifications

Immunogen

Synthetic peptide made to an internal portion of the mouse TRPM2 protein (within residues 1200-1300). [Swiss-Prot# Q91YD4]

Localization

Cell membrane.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

172 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for TRPM2 Antibody - BSA Free

Western Blot: TRPM2 AntibodyBSA Free [NB110-82364]

Western Blot: TRPM2 AntibodyBSA Free [NB110-82364]

Western Blot: TRPM2 Antibody [NB110-82364] - Detection of TRPM2 in mouse brain membrane lysates using NB110-82364.
Immunocytochemistry/ Immunofluorescence: TRPM2 Antibody - BSA Free [NB110-82364]

Immunocytochemistry/ Immunofluorescence: TRPM2 Antibody - BSA Free [NB110-82364]

Immunocytochemistry/Immunofluorescence: TRPM2 Antibody [NB110-82364] - TRPM2 antibody was tested in Neuro2A cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).

Applications for TRPM2 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50

Western Blot

2 ug/ml
Application Notes
This TRP7 antibody is useful in Immunocytochemistry/Immunofluorescence and Western blot, where a band is seen at ~172 kDa. In ICC/IF punctate membrane staining was observed in Neuro2A cells.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS and 30% Glycerol

Format

BSA Free

Preservative

0.1% Sodium Azide

Concentration

1.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: TRPM2

TRP7 (transient receptor protein 7 or TRPC7) is the seventh identified member of mammalian TRPC channel family which includes nonselective cation channels that mediate changes in plasma membrane cation permeability induced by stimulation of PLC (phospholipase C, the cellular integrator that receives input from GPCRs/ RTK). Major cellular function of TRPC channels is to regulate Ca2+ influx across plasma membrane induced by stimulation of these receptors by hormones, neurotransmitters, and growth factors. Localized in the cell membrane and nuclear envelope as multi-pass membrane protein, TRPC7 interacts with with MX1, RNF24, PRKG1 etc. and PRKG1 mediated TRPC7 phosphorylation at Thr-15 site negatively regulates TRPC7's activity. TRP7 has been suggested to form a receptor-activated non-selective calcium permeant cation channel and is operated by a phosphatidylinositol second messenger system activated by RTKs or GPCRs. TRP7 is activated by DAG (diacylglycerol) and also upon intracellular calcium store depletion.

Alternate Names

EC 3.6.1.13, EREG1MGC133383, Estrogen-responsive element-associated gene 1 protein, KNP3LTrpC-2, Long transient receptor potential channel 2, LTrpC2, LTRPC2TRPC7transient receptor potential cation channel subfamily M member 2, NUDT9H, NUDT9L1, transient receptor potential cation channel, subfamily M, member 2, Transient receptor potential channel 7, TrpC7

Gene Symbol

TRPM2

Additional TRPM2 Products

Product Documents for TRPM2 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for TRPM2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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