TRPM8 Antibody (M571)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-23237

Novus Biologicals, part of Bio-Techne
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NBP3-23237
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # M571

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all TRPM8 Antibodies »

Product Specifications

Immunogen

Clone M571 was generated from TRPM8 synthetic peptide (coupled to carrier) corresponding to amino acids in the extracellular region of human TRPM8. This site is well conserved in rat and mouse TRPM8, but has low homology to other TRPM family members. [UniProt# Q7Z2W7]

Specificity

This antibody detects a 130 kDa protein on SDS-PAGE immunoblots of human MDA-MB-231 and MCF-7 cells. The antibody is also recommended for immunocytochemical labeling of cells.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

130 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Stable for at least 1 year at -20°C.

Scientific Data Images for TRPM8 Antibody (M571)

TRPM8 Antibody (M571)

Western Blot: TRPM8 Antibody (M571) [NBP3-23237] -

Western Blot: TRPM8 Antibody (M571) [NBP3-23237] - Western blot image of human TRPM8 in human MDA-MB-231 cells. The blot was probed with mouse monoclonal anti-TRPM8 (extracellular region) clone M571 (lane 1) or clone M572 (lane 2).
TRPM8 Antibody (M571)

Immunocytochemistry/Immunofluorescence: TRPM8 Antibody (M571) [NBP3-23237] -

Immunocytochemistry/Immunofluorescence: TRPM8 Antibody (M571) [NBP3-23237] - Immunocytochemical labeling of TRPM8 in paraformaldehyde fixed and NP-40 permeabilized MCF-7 cells. The cells were labeled with mouse monoclonal anti-TRPM8 (M571). The antibody was detected using goat anti-mouse DyLight(R) 594.

Applications for TRPM8 Antibody (M571)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:200

Western Blot

1:1000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 1 mg/ml BSA and 50% glycerol

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C.

Background: TRPM8

TRPM8 is a novel gene, trp-p8 has an expression pattern within normal human tissues that is mostly restricted to prostate epithelial cells. Trp-p8 mRNA expression is at moderate levels in normal prostate tissue and appears to be elevated in prostate cancer. Notably, trp-p8 mRNA was also expressed in a number of nonprostatic primary tumors of the breast, colon, lung and skin origin, whereas transcripts encoding trp-p8 were hardly detected or not detected in the corresponding normal human tissues.

Alternate Names

CMR1, LTRPC6, TRPP8

Gene Symbol

TRPM8

Additional TRPM8 Products

Product Documents for TRPM8 Antibody (M571)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for TRPM8 Antibody (M571)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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