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TRPM8 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NB200-145

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Drosophila

Cited:

Mouse, Insect - Drosophila

Applications

Validated:

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot

Cited:

IF/IHC, Immunocytochemistry/ Immunofluorescence, In vivo assay, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1 mg/ml

Product Specifications

Immunogen

Synthetic peptides made to residues 278-292 RNQLEKYISERTIQD and the C-terminus sequence NDLKGLLKEIANKIK of the human trp-p8 protein.

Reactivity Notes

Drosophila reactivity reported in scientific literature (PMID: 19295141).

Specificity

Reacts with residues 278-292 and the C terminus sequence of the human trp-p8 protein.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

125 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for TRPM8 Antibody - BSA Free

Western Blot: TRPM8 AntibodyBSA Free [NB200-145]

Western Blot: TRPM8 AntibodyBSA Free [NB200-145]

Western Blot: TRPM8 Antibody [NB200-145] - Analysis of TRPM8 in transfected COS cells using NB200-145 at 1:750 and incubated overnight at 4C. Secondary rabbit HRP antibody was incubated 1 hour at RT followed by ECL detection.
Immunohistochemistry: TRPM8 Antibody - BSA Free [NB200-145]

Immunohistochemistry: TRPM8 Antibody - BSA Free [NB200-145]

Immunohistochemistry: TRPM8 Antibody [NB200-145] - Prostate cancer: Formalin-Fixed Paraffin-Embedded (FFPE)

Applications for TRPM8 Antibody - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Frozen

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500

Western Blot

1:500 -1:1000
Application Notes
This antibody has been tested to work in Western Blot and immunohistochemistry on paraffin and frozen sections. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: TRPM8

A novel gene, trp-p8 has an expression pattern within normal human tissues that is mostly restricted to prostate epithelial cells. Trp-p8 mRNA expression is at moderate levels in normal prostate tissue and appears to be elevated in prostate cancer. Notably, trp-p8 mRNA was also expressed in a number of nonprostatic primary tumors of the breast, colon, lung and skin origin, whereas transcripts encoding trp-p8 were hardly detected or not detected in the corresponding normal human tissues.

Alternate Names

CMR1, LTRPC6, TRPP8

Entrez Gene IDs

79054 (Human)

Gene Symbol

TRPM8

UniProt

Additional TRPM8 Products

Product Documents for TRPM8 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for TRPM8 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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