Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Validated:

Human

Applications

Immunoprecipitation, Western Blot (Negative)

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all TRRAP Antibodies »

Product Summary for TRRAP Antibody

Immunogen

The immunogen recognized by this antibody maps to a region between residue 3275 and 3325 of human transformation/transcription domain-associated protein using the numbering given in entry AAD04629.1 (GeneID 8295).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for TRRAP Antibody

Immunoprecipitation: TRRAP Antibody [NB100-58810]

Immunoprecipitation: TRRAP Antibody [NB100-58810]

Immunoprecipitation: TRRAP Antibody [NB100-58810] - Detection of Human TRRAP by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-TRRAP antibody NB100-58810 used for IP at 3 mcg/mg lysate. TRRAP was also immunoprecipitated by rabbit anti- TRRAP antibody NB100-58811, which recognizes a downstream epitope. Detection: Chemiluminescence with an exposure time of 30 seconds.

Applications for TRRAP Antibody

Application
Recommended Usage

Immunoprecipitation

2 - 5 ug/mg lysate
Application Notes
Western Blot Not recommended.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: TRRAP

The novel ATM-related protein TRRAP is an essential cofactor for the c-Myc and E2F oncoproteins. It is an adapter protein, which is found in various multiprotein chromatin complexes with histone acetyltransferase activity (HAT), which gives a specific tag for epigenetic transcription activation. TRRAP plays a central role in MYC (c-Myc) transcription activation, and also participates in cell transformation by MYC. It is required for TP53/p53-, E2F1- and E2F4-mediated transcription activation. It most likely acts by linking transcription factors such as E1A, MYC or E2F1 to HAT complexes such as STAGA thereby allowing transcription activation and it is probably not required in the steps following histone acetylation in processes of transcription activation. TRRAP may also be required for the mitotic checkpoint and normal cell cycle progression.

Long Name

Transformation/Transcription Domain-associated Protein

Alternate Names

PAF400, STAF40, TR-AP, Tra1

Entrez Gene IDs

8295 (Human)

Gene Symbol

TRRAP

UniProt

AAD04629.1 (Human)

Additional TRRAP Products

Product Documents for TRRAP Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for TRRAP Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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