Trypsin 1/PRSS1 Antibody (A16-N)

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-30135

Recombinant Monoclonal Antibody.
Novus Biologicals, part of Bio-Techne
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NBP1-30135-0.05ml
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # A16-N

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Trypsin 1/PRSS1 Antibodies »

Product Specifications

Immunogen

Peptide derived from N-terminal sequence of human Trypsin 1/PRSS1. Antibody recognizes the epitope located between Lys92 - Ans106.

Epitope

Lys92 - Ans106

Specificity

This antibody is specific for the N terminus of human pancreatic cationic trypsin.Due to the high degree of homology between human trypsins, cross-detection with other trypsins than PRSS1 is possible

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Description

This antibody is immunoaffinity purified with immunogenic peptide as a ligand.

Scientific Data Images for Trypsin 1/PRSS1 Antibody (A16-N)

Western Blot: Trypsin 1/PRSS1 Antibody (A16-N) [NBP1-30135]

Western Blot: Trypsin 1/PRSS1 Antibody (A16-N) [NBP1-30135]

Western Blot: Trypsin Antibody (A16-N) [NBP1-30135] - Trypsin-human pancreas.

Applications for Trypsin 1/PRSS1 Antibody (A16-N)

Application
Recommended Usage

ELISA

1:20000-1:50000

Western Blot

1:4000
Application Notes
For western blots, incubate the membrane with antibody diluted in blocking buffer 2 hours at room temperature. Western immunoblotting solutions: Wash buffer: 1x Tris Buffered Saline (TBS); 0.2% Tween-20. - Blocking buffer: 1xTBS; 0.2% Tween-20; 8% nonfat dry milk.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

20mM Tris/HCl (pH 8.0) and 10 mg/ml BSA

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Trypsin 1/PRSS1

This gene encodes a trypsinogen, which is a member of the trypsin family of serine proteases. This enzyme is secreted by the pancreas and cleaved to its active form in the small intestine. It is active on peptide linkages involving the carboxyl group of lysine or arginine. Mutations in this gene are associated with hereditary pancreatitis. This gene and several other trypsinogen genes are localized to the T cell receptor beta locus on chromosome 7.

Alternate Names

PRSS1, PTRYI, TRY4, Trygn16, Trypsinogen 1

Entrez Gene IDs

5644 (Human)

Gene Symbol

PRSS1

UniProt

P07477

Additional Trypsin 1/PRSS1 Products

Product Documents for Trypsin 1/PRSS1 Antibody (A16-N)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Trypsin 1/PRSS1 Antibody (A16-N)

This antibody is immunoaffinity purified with immunogenic peptide as a ligand.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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