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Trypsin 1/PRSS1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB600-1277

Novus Biologicals, part of Bio-Techne
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NB600-1277

Key Product Details

Species Reactivity

Bovine

Applications

ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit Serum

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Trypsin 1/PRSS1 1/PRSS1 [Bovine Pancreas] (Uniprot: P00760)

Reactivity Notes

Cross reactivity against Trypsin 1/PRSS1 from other tissues and species may occur but have not been specifically determined.

Localization

Secreted

Specificity

Trypsin

Clonality

Polyclonal

Host

Rabbit

Isotype

Serum

Description

This antibody was prepared from monospecific antiserum by a delipidation and defibrination. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-rabbit serum, purified and partially purified Trypsin [Bovine Pancreas]

Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.

Applications for Trypsin 1/PRSS1 Antibody

Application
Recommended Usage

ELISA

1:5000-1:25000

Western Blot

1:500-1:3000
Application Notes
This product has been assayed against 1.0 ug of Trypsin [Bovine Pancreas] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) and (ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:1,000 to 1:4,000 of the reconstitution concentration is suggested for This product . Findings were obtained using Costar Brand Vinyl Assay Plates #09997011 in NaHCO3 buffer at pH 8.0.

Formulation, Preparation, and Storage

Purification

Delipidation and Defibrination

Formulation

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Preservative

0.01% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Trypsin 1/PRSS1

The Trypsin gene encodes a trypsinogen, which is a member of the trypsin family of serine proteases. This enzyme is secreted by the pancreas and cleaved to its active form in the small intestine. It is active on peptide linkages involving the carboxyl group of l

Alternate Names

PRSS1, PTRYI, TRY4, Trygn16, Trypsinogen 1

Gene Symbol

PRSS1

UniProt

Additional Trypsin 1/PRSS1 Products

Product Documents for Trypsin 1/PRSS1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Trypsin 1/PRSS1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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