Tryptase alpha/beta 1 Antibody (TPSAB1/1961) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-75754
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Protein Array
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # TPSAB1/1961
Format
Azide and BSA Free
Concentration
1 mg/ml
Product Summary for Tryptase alpha/beta 1 Antibody (TPSAB1/1961) - Azide and BSA Free
Immunogen
Recombinant human Tryptase alpha/TPS1 protein fragment (around aa 115-233) (Exact sequence is proprietary) (Uniprot: Q15661)
Localization
Secreted and Cytoplasmic
Specificity
This antibody reacts with mast cells distributed in skin, synovium, lung, and heart. This antibody does not bind with any other cell type. Human mast cell tryptase is considered to be an important marker of mast cell activation and is an important mediator of inflammation. Mastocytosis is a term collectively used for a group of disorders in which there is abnormal accumulation of mast cells in one or multiple organs. Anti-tryptase, combined with anti-CD2, anti-CD25, and anti-CD117, can be useful in identifying reactive mast cell hyperplasia, myelogenous neoplasms, mast cell leukemia, and mastocytosis.Due to sequence identity this antibody will likely cross react with tryptase beta 2.
Marker
Mast Cell Marker
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Description
1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-75751).
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Scientific Data Images for Tryptase alpha/beta 1 Antibody (TPSAB1/1961) - Azide and BSA Free
Immunohistochemistry-Paraffin: Tryptase alpha/beta 1 Antibody (TPSAB1/1961) - Azide and BSA Free [NBP2-75754]
Immunohistochemistry-Paraffin: Tryptase alpha/TPS1 Antibody (TPSAB1/1961) - Azide and BSA Free [NBP2-75754] - Formalin-fixed, paraffin-embedded human Tonsil stained with Tryptase Mouse Monoclonal Antibody (TPSAB1/1961).Protein Array: Tryptase alpha/beta 1 Antibody (TPSAB1/1961)Azide and BSA Free [NBP2-75754]
Protein Array: Tryptase alpha/TPS1 Antibody (TPSAB1/1961) - Azide and BSA Free [NBP2-75754] - Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (mAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a mAb to its intended target. A mAbis considered to specific to its intended target, if the mAb has an S-score of at least 2.5. For example, if a mAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that mAb to protein X is equal to 29.Applications for Tryptase alpha/beta 1 Antibody (TPSAB1/1961) - Azide and BSA Free
Application
Recommended Usage
Immunohistochemistry
1 - 2 ug/mL
Immunohistochemistry-Paraffin
1 - 2 ug/mL
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Background: Tryptase alpha/beta 1
Alternate Names
alpha-Tryptase, EC:3.4.21.59, epididymis secretory sperm binding protein, mast cell alpha II tryptase, mast cell beta I tryptase, mast cell tryptase, TPS1, TPS2, TPSAB1, TPSB1, TPSB2, Tryptase 1, tryptase alpha II, Tryptase alpha-1, tryptase beta I, tryptase beta-1, Tryptase beta-2, Tryptase I, tryptase II, tryptase-1, tryptase-2, tryptase-I, tryptase-III
Gene Symbol
TPSAB1
UniProt
Additional Tryptase alpha/beta 1 Products
Product Documents for Tryptase alpha/beta 1 Antibody (TPSAB1/1961) - Azide and BSA Free
Product Specific Notices for Tryptase alpha/beta 1 Antibody (TPSAB1/1961) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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