Tryptophan Hydroxylase 1/TPH-1 [p Ser58] Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NB300-260
![Western Blot: Tryptophan Hydroxylase 1/TPH-1 [p Ser58] Antibody [NB300-260]](https://resources.bio-techne.com/images/products/Tryptophan-Hydroxylase-1-%5Bp-Ser58%5D-Antibody-Western-Blot-NB300-260-img0002.jpg "Western Blot: Tryptophan Hydroxylase 1/TPH-1 [p Ser58] Antibody [NB300-260]")
Key Product Details
Species Reactivity
Validated:
Mouse, Rabbit
Predicted:
Bovine (100%), Canine (100%), Chicken (100%), Human (100%), Mouse (100%), Primate (100%), Rat (100%), Xenopus (100%), Zebrafish (100%). Backed by our 100% Guarantee.
Applications
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for Tryptophan Hydroxylase 1/TPH-1 [p Ser58] Antibody
Immunogen
Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser58 conjugated to KLH. Accession # P09810
Modification
p Ser58
Localization
Cytoplasmic
Specificity
Specific for endogenous levels of the ~55 kDa Tryptophan Hydroxylase 1/TPH-1 protein phosphorylated at Ser58.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
55 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Tryptophan Hydroxylase 1/TPH-1 [p Ser58] Antibody
![Western Blot: Tryptophan Hydroxylase 1/TPH-1 [p Ser58] Antibody [NB300-260]](https://resources.bio-techne.com/images/products/Tryptophan-Hydroxylase-1-[p-Ser58]-Antibody-Western-Blot-NB300-260-img0002.jpg "Western Blot: Tryptophan Hydroxylase 1/TPH-1 [p Ser58] Antibody [NB300-260]")
Western Blot: Tryptophan Hydroxylase 1/TPH-1 [p Ser58] Antibody [NB300-260]
Western Blot: Tryptophan Hydroxylase 1 [p Ser58] Antibody [NB300-260] - Recombinant tryptophan hydroxylase incubated in the absence (Control) and presence of cAMP-dependent protein kinase (PKA) showing specific immunolabeling of the ~53k tryptophan hydroxylase protein phosphorylated at Ser58.Applications for Tryptophan Hydroxylase 1/TPH-1 [p Ser58] Antibody
Application
Recommended Usage
Western Blot
1:1000
Application Notes
Antibody dilutions and tissue load should be based on tissue type and expected phosphorylation state expression levels.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
10mM HEPES (pH 7.5), 0.15M NaCl, 0.1 mg/ml BSA and 50% Glycerol
Preservative
No Preservative
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Tryptophan Hydroxylase 1/TPH-1
Additional Tryptophan Hydroxylase 1/TPH-1 Products
Product Documents for Tryptophan Hydroxylase 1/TPH-1 [p Ser58] Antibody
Product Specific Notices for Tryptophan Hydroxylase 1/TPH-1 [p Ser58] Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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