Tryptophan Hydroxylase 1/TPH-1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-86922

Novus Biologicals, part of Bio-Techne
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NBP1-86922
NBP1-86922-25ul
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Tryptophan Hydroxylase 1/TPH-1 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: ISELKHALSGHAKVKPFDPKITCKQECLITTFQDVYFVSESFEDAKEKMREFTKTIKRPFGVKYNPYTRSIQILKDTKSITSAMNELQHDLDVVSDALAKVSRKPSI

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (85%), Rat (86%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Tryptophan Hydroxylase 1/TPH-1 Antibody

Immunohistochemistry-Paraffin: Tryptophan Hydroxylase 1/TPH-1 Antibody [NBP1-86922]

Immunohistochemistry-Paraffin: Tryptophan Hydroxylase 1/TPH-1 Antibody [NBP1-86922]

Immunohistochemistry-Paraffin: Tryptophan Hydroxylase 1/TPH-1 Antibody [NBP1-86922] - Staining in human small intestine and liver tissues . Corresponding TPH1 RNA-seq data are presented for the same tissues.
Western Blot: Tryptophan Hydroxylase 1/TPH-1 Antibody [NBP1-86922]

Western Blot: Tryptophan Hydroxylase 1/TPH-1 Antibody [NBP1-86922]

Western Blot: Tryptophan Hydroxylase 1/TPH-1 Antibody [NBP1-86922] - Human melanoma cell line A375 and 5 patient melanoma tissue samples. 15 ug cell lysate per lane. TPH1 antibody at 1:400, 4C O/N. WB image submitted by a verified customer review.
Immunohistochemistry-Paraffin: Tryptophan Hydroxylase 1/TPH-1 Antibody [NBP1-86922]

Immunohistochemistry-Paraffin: Tryptophan Hydroxylase 1/TPH-1 Antibody [NBP1-86922]

Immunohistochemistry-Paraffin: Tryptophan Hydroxylase 1/TPH-1 Antibody [NBP1-86922] - Staining of human small intestine shows moderate to strong cytoplasmic positivity in glandular cells.
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Applications for Tryptophan Hydroxylase 1/TPH-1 Antibody

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

Validated from a verified customer review
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 5 using NBP1-86922 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Tryptophan Hydroxylase 1/TPH-1

Tryptophan hydroxylase (TPH) is the rate-limiting enzyme in the biosynthesis of serotonin (5-HT) and might be related to the pathogenesis of major depression (MD). Two isoforms are known, TPH1 and TPH2. (1). TPH1 is phosphorylated by cAMP-dependent protein kinase A (2). Recently, it has been shown that expression of the TPH1 gene is increased in lungs and pulmonary endothelial cells from patients with idiopathic pulmonary arterial hypertension. Results indicate that TPH1 and peripheral serotonin play an essential role in the development of hypoxia-induced elevations in pulmonary pressures and hypoxia-induced pulmonary vascular remodeling. In addition, the results suggest that, in mice, serotonin has differential effects on the pulmonary vasculature and right ventricular hypertrophy (3).

Alternate Names

TPH-1, TPRH

Gene Symbol

TPH1

Additional Tryptophan Hydroxylase 1/TPH-1 Products

Product Documents for Tryptophan Hydroxylase 1/TPH-1 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Tryptophan Hydroxylase 1/TPH-1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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