Tryptophan hydroxylase 2 Antibody (PSH04-89)

Immunohistochemistry-Frozen: Tryptophan hydroxylase 2 Antibody (PSH04-89) [NBP3-33037]

Immunohistochemistry-Frozen: Tryptophan hydroxylase 2 Antibody (PSH04-89) [NBP3-33037] - Immunofluorescence analysis of frozen mouse brain (raphe nucleus) tissue with Rabbit anti-Tryptophan hydroxylase 2 antibody (NBP3-33037) at 1/200 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for about 2 minutes in microwave oven. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-33037, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).

Western Blot: Tryptophan hydroxylase 2 Antibody (PSH04-89) [NBP3-33037]

Western Blot: Tryptophan hydroxylase 2 Antibody (PSH04-89) [NBP3-33037] - Western blot analysis of Tryptophan hydroxylase 2 on different lysates with Rabbit anti-Tryptophan hydroxylase 2 antibody (NBP3-33037) at 1/1,000 dilution.

Lane 1: Mouse hippocampus tissue lysate
Lane 2: Mouse brain tissue lysate
Lane 3: Rat brain tissue lysate
Lane 4: Rat hippocampus tissue lysate

Lysates/proteins at 30 ug/Lane.

Predicted band size: 56 kDa
Observed band size: 50 kDa

Exposure time: 1 minute 18 seconds; ECL;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33037) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.

Immunohistochemistry: Tryptophan hydroxylase 2 Antibody (PSH04-89) [NBP3-33037]

Immunohistochemistry: Tryptophan hydroxylase 2 Antibody (PSH04-89) [NBP3-33037] - Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-Tryptophan hydroxylase 2 antibody (NBP3-33037) at 1/500 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-33037) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.