Tryptophan hydroxylase 2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-2447

Novus Biologicals, part of Bio-Techne
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NB100-2447
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Predicted:

Bovine (100%), Canine (100%), Porcine (100%). Backed by our 100% Guarantee.

Applications

Validated:

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA

Cited:

Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all Tryptophan Hydroxylase 2 Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-NKPNSGKNDDKGNK corresponding to internal region according to NP_775489.2.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Tryptophan hydroxylase 2 Antibody

Immunocytochemistry/ Immunofluorescence: Tryptophan hydroxylase 2 Antibody [NB100-2447]

Immunocytochemistry/ Immunofluorescence: Tryptophan hydroxylase 2 Antibody [NB100-2447]

Immunocytochemistry/Immunofluorescence: Tryptophan hydroxylase 2 Antibody [NB100-2447] - Immunofluorescence analysis of paraformaldehyde fixed A549 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
Immunohistochemistry-Paraffin: Tryptophan hydroxylase 2 Antibody [NB100-2447]

Immunohistochemistry-Paraffin: Tryptophan hydroxylase 2 Antibody [NB100-2447]

Immunohistochemistry-Paraffin: Tryptophan hydroxylase 2 Antibody [NB100-2447] - Staining of paraffin embedded Human Brain Stem. Antibody at 2 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.
Flow Cytometry: Tryptophan hydroxylase 2 Antibody [NB100-2447]

Flow Cytometry: Tryptophan hydroxylase 2 Antibody [NB100-2447]

Flow Cytometry: Tryptophan hydroxylase 2 Antibody [NB100-2447] - Flow cytometric analysis of paraformaldehyde fixed U251 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
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Applications for Tryptophan hydroxylase 2 Antibody

Application
Recommended Usage

Flow Cytometry

10 ug/mL

Immunocytochemistry/ Immunofluorescence

10 ug/mL

Immunohistochemistry

2 - 4 ug/mL

Immunohistochemistry-Paraffin

2 - 4 ug/mL

Peptide ELISA

Detection limit 1:32000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Tryptophan Hydroxylase 2

Tryptophan hydroxylase (TPH) catalyzes the 5-hydroxylation of tryptophan, which is the first step in the biosynthesis of indoleamines (serotonin and melatonin) (Martinez et al., 2001). In mammals, serotonin biosynthesis occurs predominantly in neurons which originate in the Raphe nuclei of the brain, and melatonin synthesis takes place within the pineal gland. Although TPH catalyzes the same reaction within the Raphe nuclei and the pineal gland, TPH activity is rate-limiting for serotonin but not melatonin biosynthesis. Serotonin functions mainly as a neurotransmitter, whereas melatonin is the principal hormone secreted by the pineal gland. The activity of TPH is enhanced by phosphorylation by cAMP-dependent protein kinase (PKA) and Ca2+/calmodulin kinase II (CaM K II) (Jiang et al., 2000; Johansen et al., 1996). CaM K II phosphorylates Ser260 which lies within the regulatory domain of TPH (Jiang et al., 2000).

Alternate Names

NTPH, TPH2

Entrez Gene IDs

121278 (Human)

Gene Symbol

TPH2

UniProt

NP_775489.2 (Human)

Additional Tryptophan Hydroxylase 2 Products

Product Documents for Tryptophan hydroxylase 2 Antibody

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Product Specific Notices for Tryptophan hydroxylase 2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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