Tryptophan hydroxylase 2 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NB100-74555

Novus Biologicals, part of Bio-Techne
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NB100-74555
NB100-74555-0.025ml
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse, Rat, Primate, Rabbit

Cited:

Human, Mouse, Rat, Primate

Applications

Validated:

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry Free-Floating, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Cited:

Block/Neutralize, Flow Cytometry, IF/IHC, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry Free-Floating, Immunohistochemistry-Frozen, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1.0 mg/ml

View all Tryptophan Hydroxylase 2 Antibodies »

Product Specifications

Immunogen

Synthetic peptide make to an internal portion of the mouse Tryptophan hydroxylase 2 protein (between amino acids 10-75) [UniProt Q8CGV2]

Reactivity Notes

Human reactivity is weak. Mouse reactivity reported in scientific literature (PMID: 16581041). Rat reactivity reported in scientific literature (PMID: 24066056).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Tryptophan hydroxylase 2 Antibody - BSA Free

Immunohistochemistry-Paraffin: Tryptophan hydroxylase 2 Antibody - BSA Free [NB100-74555]

Immunohistochemistry-Paraffin: Tryptophan hydroxylase 2 Antibody - BSA Free [NB100-74555]

Immunohistochemistry-Paraffin: Tryptophan hydroxylase 2 Antibody [NB100-74555] - IHC analysis of a formalin fixed paraffin embedded tissue section of mouse brain using Tryptophan hydroxylase 2 antibody at 1:25 dilution. The staining was developed using HRP-DAB detection method and the nuclei were counterstained with hematoxylin. The antibody generated staining of the neurons.
Western Blot: Tryptophan hydroxylase 2 AntibodyBSA Free [NB100-74555]

Western Blot: Tryptophan hydroxylase 2 AntibodyBSA Free [NB100-74555]

Western Blot: Tryptophan hydroxylase 2 Antibody [NB100-74555] - Total protein from mouse brain, stomach and human brain was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-Tryptophan Hydroxylase 2 in 1% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.
Immunocytochemistry/ Immunofluorescence: Tryptophan hydroxylase 2 Antibody - BSA Free [NB100-74555]

Immunocytochemistry/ Immunofluorescence: Tryptophan hydroxylase 2 Antibody - BSA Free [NB100-74555]

Immunocytochemistry/Immunofluorescence: Tryptophan hydroxylase 2 Antibody [NB100-74555] - The 5-HT and Tryptophan hydroxylase 2 in the raphe nuclei of the brain. The 5-HT- and Tryptophan hydroxylase 2-positive double stained cells in the dorsal raphe nuclei. Representative photomicrographs were taken at magnifications of 100x. Quantification of 5-HT and Tryptophan hydroxylase 2. Image collected and cropped by CiteAb from the following publication (https://www.mdpi.com/2218-273X/10/1/71), licensed under a CC-BY license.
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Applications for Tryptophan hydroxylase 2 Antibody - BSA Free

Application
Recommended Usage

Flow Cytometry

reported in scientific literature (PMID 30581079)

Immunocytochemistry/ Immunofluorescence

1:500

Immunohistochemistry

1:10-1:500

Immunohistochemistry Free-Floating

reported in scientific literature (PMID 26428905)

Immunohistochemistry-Frozen

reported in scientific literature (PMID 30350781)

Immunohistochemistry-Paraffin

1:100

Immunoprecipitation

1:1000

Western Blot

1:1000
Application Notes
IF usage was reported in scientific literature (see Soiza-Reillly M et al, 2011).

Reviewed Applications

Read 3 reviews rated 4.7 using NB100-74555 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Tryptophan Hydroxylase 2

Tryptophan hydroxylase (TPH) catalyzes the 5-hydroxylation of tryptophan, which is the first step in the biosynthesis of indoleamines (serotonin and melatonin) (Martinez et al., 2001). In mammals, serotonin biosynthesis occurs predominantly in neurons which originate in the Raphe nuclei of the brain, and melatonin synthesis takes place within the pineal gland. Although TPH catalyzes the same reaction within the Raphe nuclei and the pineal gland, TPH activity is rate-limiting for serotonin but not melatonin biosynthesis. Serotonin functions mainly as a neurotransmitter, whereas melatonin is the principal hormone secreted by the pineal gland. The activity of TPH is enhanced by phosphorylation by cAMP-dependent protein kinase (PKA) and Ca2+/calmodulin kinase II (CaM K II) (Jiang et al., 2000; Johansen et al., 1996). CaM K II phosphorylates Ser260 which lies within the regulatory domain of TPH (Jiang et al., 2000).

Alternate Names

NTPH, TPH2

Entrez Gene IDs

121278 (Human); 216343 (Mouse); 317675 (Rat)

Gene Symbol

TPH2

UniProt

Q8IWU9 (Human)

Additional Tryptophan Hydroxylase 2 Products

Product Documents for Tryptophan hydroxylase 2 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Tryptophan hydroxylase 2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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