Tryptophanyl tRNA synthetase Antibody (3A12)

Novus Biologicals, part of Bio-Techne | Catalog # H00007453-M02

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 3A12

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Tryptophanyl tRNA synthetase Antibodies »

Product Specifications

Immunogen

WARS (NP_004175, 50 a.a. ~ 149 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. YKAAAGEDYKADCPPGNPAPTSNHGPDATEAEEDFVDPWTVQTSSAKGIDYDKLIVRFGSSKIDKELINRIERATGQRPHHFLRRGIFFSHRDMNQVLDA

Specificity

WARS (3A12)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for Tryptophanyl tRNA synthetase Antibody (3A12)

Western Blot: Tryptophanyl tRNA synthetase Antibody (3A12) [H00007453-M02]

Western Blot: Tryptophanyl tRNA synthetase Antibody (3A12) [H00007453-M02]

Western Blot: Tryptophanyl tRNA synthetase Antibody (3A12) [H00007453-M02] - WARS monoclonal antibody (M02), clone 3A12 Analysis of WARS expression in HeLa.
Immunocytochemistry/ Immunofluorescence: Tryptophanyl tRNA synthetase Antibody (3A12) [H00007453-M02]

Immunocytochemistry/ Immunofluorescence: Tryptophanyl tRNA synthetase Antibody (3A12) [H00007453-M02]

Immunocytochemistry/Immunofluorescence: Tryptophanyl tRNA synthetase Antibody (3A12) [H00007453-M02] - Analysis of monoclonal antibody to WARS on HeLa cell. Antibody concentration 10 ug/ml.
Western Blot: Tryptophanyl tRNA synthetase Antibody (3A12) [H00007453-M02]

Western Blot: Tryptophanyl tRNA synthetase Antibody (3A12) [H00007453-M02]

Western Blot: Tryptophanyl tRNA synthetase Antibody (3A12) [H00007453-M02] - Analysis of WARS expression in transfected 293T cell line by WARS monoclonal antibody (M02), clone 3A12.Lane 1: WARS transfected lysate(53.2 KDa).Lane 2: Non-transfected lysate.
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Applications for Tryptophanyl tRNA synthetase Antibody (3A12)

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunoprecipitation

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IF and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Tryptophanyl tRNA synthetase

Aminoacyl-tRNA synthetases catalyze the aminoacylation of tRNA by their cognate amino acid. Because of their central role in linking amino acids with nucleotide triplets contained in tRNAs, aminoacyl-tRNA synthetases are thought to be among the first proteins that appeared in evolution. Two forms of tryptophanyl-tRNA synthetase exist, a cytoplasmic form, named WARS, and a mitochondrial form, named WARS2. Tryptophanyl-tRNA synthetase (WARS) catalyzes the aminoacylation of tRNA(trp) with tryptophan and is induced by interferon. Tryptophanyl-tRNA synthetase belongs to the class I tRNA synthetase family. Four transcript variants encoding two different isoforms have been found for this gene.

Alternate Names

EC 6.1.1, EC 6.1.1.2, GAMMA-2, hWRS, IFI53, IFP53trpRS, Interferon-induced protein 53, TrpRS, tryptophan tRNA ligase 1, cytoplasmic, Tryptophan--tRNA ligase, tryptophanyl-tRNA synthetase, tryptophanyl-tRNA synthetase, cytoplasmic, WRS

Entrez Gene IDs

7453 (Human)

Gene Symbol

WARS1

OMIM

191050 (Human)

UniProt

NP_004175 (Human)

Additional Tryptophanyl tRNA synthetase Products

Product Documents for Tryptophanyl tRNA synthetase Antibody (3A12)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Tryptophanyl tRNA synthetase Antibody (3A12)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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