Key Product Details

Species Reactivity

Human, Mouse

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all TSC1 Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 1100 and the C-terminus (residue 1164) of human tuberous sclerosis 1 using the numbering given in Swiss-Prot entry Q92574 (GeneID 7248).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for TSC1 Antibody

Western Blot: TSC1 Antibody [NB100-2315]

Western Blot: TSC1 Antibody [NB100-2315]

Western Blot: TSC1 Antibody [NB100-2315] - Detection of Human and Mouse TSC1 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified goat anti-TSC1 antibody NB100-2315 used for WB at 0.05 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Western Blot: TSC1 Antibody [NB100-2315]

Western Blot: TSC1 Antibody [NB100-2315]

Western Blot: TSC1 Antibody [NB100-2315] - Detection of Human and Mouse TSC1 on HeLa whole cell lysate using NB100-2315.

Applications for TSC1 Antibody

Application
Recommended Usage

Immunoprecipitation

1-5 ug/mg lysate

Western Blot

1:5000-1:25000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: TSC1

The mammalian Target of Rapamycin (TOR, also known as mTOR) is an evolutionarily conserved serine/threonine kinase that regulates cell growth and cell cycle progression through its ability to integrate signals from nutrient levels and growth factors. TOR regulation is accomplished through a network of various activators and repressors. It is phosphorylated by Akt, whose activity is indirectly inhibited by the lipid phosphatase PTEN. TOR is normally associated with the regulatory proteins RAPTOR, a scaffold protein whose binding by TOR substrates is necessary for effective TOR-catalyzed phosphorylation, and GBetaL, which stimulates TOR's kinase activity towards downstream proteins. It is further regulated by the proteins Rheb, TSC1 and TSC2, which act to modulate TOR activity. The downstream targets of TOR are thought to be the ribosomal protein S6 kinases and the eukaryotic initiation factor 4E binding proteins (4EBPs) whose activation leads to increased protein translation and cell growth.

Long Name

Tuberous Sclerosis 1

Alternate Names

Hamartin

Entrez Gene IDs

7248 (Human); 64930 (Mouse)

Gene Symbol

TSC1

UniProt

Q92574

Additional TSC1 Products

Product Documents for TSC1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for TSC1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.