TSC22/TSC22D1 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-98963
![Western Blot: TSC22/TSC22D1 Antibody [NBP2-98963]](https://resources.bio-techne.com/images/products/TSC22-TSC22D1-Antibody-Western-Blot-NBP2-98963-img0002.jpg "Western Blot: TSC22/TSC22D1 Antibody [NBP2-98963]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Predicted:
Mouse (100%). Backed by our 100% Guarantee.
Applications
Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Produced in rabbits immunized with a synthetic peptide corresponding to the N-terminus of the Human TSC22/TSC22D1.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
Scientific Data Images for TSC22/TSC22D1 Antibody
Western Blot: TSC22/TSC22D1 Antibody [NBP2-98963]
Western Blot: TSC22/TSC22D1 Antibody [NBP2-98963] - Anti-TSC22/TSC22D1 rabbit polyclonal antibody at 1:500 dilution. Lane A: MCF7 Whole Cell Lysate Lane B: HepG2 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 110 kDa. Observed band size: 120 kDa (We are unsure as to the identity of these extra bands).![Immunoprecipitation: TSC22/TSC22D1 Antibody [NBP2-98963]](https://resources.bio-techne.com/images/products/TSC22-TSC22D1-Antibody-Immunoprecipitation-NBP2-98963-img0001.jpg "Immunoprecipitation: TSC22/TSC22D1 Antibody [NBP2-98963]")
Immunoprecipitation: TSC22/TSC22D1 Antibody [NBP2-98963]
Immunoprecipitation: TSC22/TSC22D1 Antibody [NBP2-98963] - TSC22/TSC22D1 was immunoprecipitated using: Lane A: 0.5 mg HepG2 Whole Cell Lysate Lane B: 0.5 mg Jurkat Whole Cell Lysate 4 ul anti-TSC22/TSC22D1 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-TSC22/TSC22D1 rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 110 kDa. Observed band size: 110 kDaApplications for TSC22/TSC22D1 Antibody
Application
Recommended Usage
Immunoprecipitation
1-4 uL/mg of lysate
Western Blot
1:500-1:2000
Formulation, Preparation, and Storage
Purification
Antigen and protein A Affinity-purified
Formulation
0.2 um filtered solution in PBS
Preservative
No Preservative
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: TSC22
Long Name
TSC22 Domain Family, Member 1
Alternate Names
Egr5, TGFB1I4, TSC22D1
Gene Symbol
TSC22D1
Additional TSC22 Products
Product Documents for TSC22/TSC22D1 Antibody
Product Specific Notices for TSC22/TSC22D1 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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