TSLP Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NB110-55234

Novus Biologicals, part of Bio-Techne
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NB110-55234
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Key Product Details

Validated by

Tagged Protein Expression, Biological Validation

Species Reactivity

Validated:

Human

Cited:

Human, Mouse

Applications

Validated:

ELISA, Immunocytochemistry/ Immunofluorescence, Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

IF/IHC, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1.0 mg/ml

View all TSLP Antibodies »

Product Specifications

Immunogen

Antibody was raised against a peptide corresponding to 19 amino acids near the center of human TSLP. The immunogen is located within the amino acids 40-90 of TSLP. Amino Acid Sequence: SGTKSTEFNNTVSCSNRPH

Specificity

Human TSLP has 2 isoforms, including isoform 1 (159aa, 18kD) and isoform 2 (63aa, 7kD). Mouse TSLP has one isoform (140aa, 16kD) and Rat TSLP also has one isoform (135aa, 16kD). NB110-55234 can detect only longer human isoform, but cannot detect shorter human isoform, mouse and rat.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

18 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for TSLP Antibody - BSA Free

Western Blot: TSLP AntibodyBSA Free [NB110-55234]

Western Blot: TSLP AntibodyBSA Free [NB110-55234]

Western Blot: TSLP Antibody [NB110-55234] - EGF attenuates protein expression of PAR-2 and TSLP in DNCB-induced AD-like skin lesions in NC/Nga mice. Lysates were immunoblotted with anti-phospho-ERK, anti-ERK, anti-PAR-2, anti-TSLP, and anti-beta-actin antibodies. Cropped images from the different parts were displayed. Image collected and cropped by CiteAb from the following publication (www.nature.com/articles/s41598-018-30404-x) licensed under a CC-BY license.
Immunohistochemistry: TSLP Antibody - BSA Free [NB110-55234]

Immunohistochemistry: TSLP Antibody - BSA Free [NB110-55234]

Immunohistochemistry: TSLP Antibody [NB110-55234] - EGF attenuates protein expression of PAR-2 and TSLP in DNCB-induced AD-like skin lesions in NC/ Nga mice. Paraffin sections of skin biopsies from DNCB-induced AD-like skin lesions were immunostained with anti-PAR-2 and anti-TSLP antibodies. Dark brown dots (Te red arrows) indicate positively stained cells. Images are representatives of groups. Scale bar, 100 um. Image collected and cropped by CiteAb from the following publication (www.nature.com/articles/s41598-018-30404-x) licensed under a CC-BY license.
Western Blot: TSLP AntibodyBSA Free [NB110-55234]

Western Blot: TSLP AntibodyBSA Free [NB110-55234]

Western Blot: TSLP Antibody [NB110-55234] - Total protein levels of TSLP in patients with AERD and subjects with CRS measured by using Western blotting of 3 representative samples per group. This image was submitted via customer Review.
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Applications for TSLP Antibody - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

20 ug/mL

Immunofluorescence

20 ug/mL

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

2.5 ug/ml

Western Blot

0.25-4 ug/ml

Reviewed Applications

Read 1 review rated 5 using NB110-55234 in the following applications:

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: TSLP

Thymic stromal lymphopoietin (TSLP) has recently been identified as an important factor capable of driving dendritic cell maturation and activation. TSLP is a four-helix-bundle cytokine that is expressed mainly by barrier epithelial cells and is a potent activator of several cell types such as myeloid dendritic cells. TSLP is involved in the positive selection of regulatory T cells, maintenance of peripheral CD4+ T cell homeostasis and the induction of CD4+ T cell-mediated allergic reaction. TSLP is also capable of supporting the growth of fetal liver and adult B cell progenitors and their differentiation to the IgM-positive stage of B cell development. Amino acid sequence analysis has shown poor homology between human and mouse TSLP although they exhibit similar biological functions and are expressed in similar tissues. Despite its predicted molecular weight, TSLP often migrates at a higher molecular weight in SDS-PAGE. At least two differentially spliced isoforms of TSLP are known to exist.

Long Name

Thymic Stromal Lymphopoietin

Alternate Names

thymic stromal lymphopoietin

Entrez Gene IDs

85480 (Human); 53603 (Mouse)

Gene Symbol

TSLP

UniProt

Q969D9

Additional TSLP Products

Product Documents for TSLP Antibody - BSA Free

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Product Specific Notices for TSLP Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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