Tsukushi/TSK Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-87959

Novus Biologicals, part of Bio-Techne
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NBP1-87959
NBP1-87959-25ul
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Knockdown Validated, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Tsukushi/TSK Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: DTAHLDLSSNRLEMVNESVLAGPGYTTLAGLDLSHNLLTSISPTAFSRLRYLESLDLSHNGLTALPAESFTSSPLSDVNLSHNQLREVSVSAFTTHSQGRALHVDLSHNLIHR

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Tsukushi/TSK Antibody

Western Blot: Tsukushi/TSK Antibody [NBP1-87959]

Western Blot: Tsukushi/TSK Antibody [NBP1-87959]

Western Blot: Tsukushi/TSK Antibody [NBP1-87959] - Analysis in A-549 cells transfected with control siRNA, target specific siRNA probe #1,. Remaining relative intensity is presented. Loading control: Anti-PPIB.
Immunocytochemistry/ Immunofluorescence: Tsukushi/TSK Antibody [NBP1-87959]

Immunocytochemistry/ Immunofluorescence: Tsukushi/TSK Antibody [NBP1-87959]

Immunocytochemistry/Immunofluorescence: Tsukushi/TSK Antibody [NBP1-87959] - Staining of human cell line A549 shows localization to nucleus. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: Tsukushi/TSK Antibody [NBP1-87959]

Immunohistochemistry-Paraffin: Tsukushi/TSK Antibody [NBP1-87959]

Immunohistochemistry-Paraffin: Tsukushi/TSK Antibody [NBP1-87959] - Staining of human kidney shows moderate cytoplasmic positivity in cells in tubules.
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Applications for Tsukushi/TSK Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Tsukushi/TSK

Tsukushi (TSK) is a secreted member of the SLRP (small leucine-rich proteoglycan) family of extracellular matrix molecules. It is named after a Japanese horsetail plant, which its expression pattern resembles in the chick primitive streak. Tsukushi binds BMP and Delta-1, contributes to formation of the primitive organizer and neural crest, and is upregulated in certain breast and bone cancer cell lines.

Long Name

Tsukushin Leucine-rich Repeat Containing Protein 54

Alternate Names

E2IG4, LRRC54, TSKU

Gene Symbol

TSKU

Additional Tsukushi/TSK Products

Product Documents for Tsukushi/TSK Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Tsukushi/TSK Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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