TTC11 Antibody (6S1G4)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15848
Recombinant Monoclonal Antibody
Key Product Details
Validated by
Knockout/Knockdown
Species Reactivity
Human, Mouse, Rat
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Knockout Validated, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 6S1G4
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-122 of human TTC11 (NP_057152.2). MEAVLNELVSVEDLLKFEKKFQSEKAAGSVSKSTQFEYAWCLVRSKYNDDIRKGIVLLEELLPKGSKEEQRDYVFYLAVGNYRLKEYEKALKYVRGLLQTEPQNNQAKELERLIDKAMKKDG
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for TTC11 Antibody (6S1G4)
Western Blot: TTC11 Antibody (6S1G4) [NBP3-15848]
Western Blot: TTC11 Antibody (6S1G4) [NBP3-15848] - Western blot analysis of extracts of various cell lines, using TTC11 antibody (NBP3-15848) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.Immunocytochemistry/ Immunofluorescence: TTC11 Antibody (6S1G4) [NBP3-15848]
Immunocytochemistry/Immunofluorescence: TTC11 Antibody (6S1G4) [NBP3-15848] - Immunofluorescence analysis of NIH-3T3 cells using TTC11 antibody (NBP3-15848). Blue: DAPI for nuclear staining.Immunohistochemistry-Paraffin: TTC11 Antibody (6S1G4) [NBP3-15848]
Immunohistochemistry-Paraffin: TTC11 Antibody (6S1G4) [NBP3-15848] - Immunohistochemistry of paraffin-embedded mouse kidney using TTC11 antibody (NBP3-15848) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.Applications for TTC11 Antibody (6S1G4)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:50 - 1:200
Immunohistochemistry
1:50 - 1:200
Immunoprecipitation
0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Western Blot
1:500 - 1:1000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.3), 50% glycerol
Preservative
0.05% Proclin 300
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: TTC11
Alternate Names
CGI-135, Fis1, FIS1 homolog, fission 1 (mitochondrial outer membrane) homolog (S. cerevisiae), fission 1 (mitochondrial outer membrane) homolog (yeast), H_NH0132A01.6, hFis1, mitochondrial fission 1 protein, tetratricopeptide repeat domain 11, Tetratricopeptide repeat protein 11, TTC11TPR repeat protein 11
Gene Symbol
FIS1
Additional TTC11 Products
Product Documents for TTC11 Antibody (6S1G4)
Product Specific Notices for TTC11 Antibody (6S1G4)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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⚠ WARNING: This product can expose you to chemicals including Methotrexate, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov