TTF-1 / NKX2-1 Antibody (TTF1/8123R)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-20231

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Kappa Clone # TTF1/8123R

Concentration

0.2 mg/ml

View all TTF-1/NKX2-1 Antibodies »

Product Summary for TTF-1 / NKX2-1 Antibody (TTF1/8123R)

Immunogen

Recombinant fragment (around aa1-400) of human TTF-1 / NKX2-1 protein (exact sequence is proprietary)

Localization

Nucleus.

Specificity

Anti-TTF-1 is useful in differentiating primary adenocarcinoma of the lung from metastatic carcinomas originating in the breast, mediastinal germ cell tumors, and malignant mesothelioma It can also be used to differentiate small cell lung carcinoma from lymphoid infiltrates

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG Kappa

Description

Positive Controls: Normal thyroid or lung.

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. Non-hazardous. No MSDS required.

Scientific Data Images for TTF-1 / NKX2-1 Antibody (TTF1/8123R)

TTF-1 / NKX2-1 Antibody (TTF1/8123R)

TTF-1 / NKX2-1 Antibody (TTF1/8123R)

Formalin-fixed, paraffin-embedded human lung adenocarcinoma stained with TTF-1 / NKX2-1 antibody (TTF1/8123R). HIER: Tris/EDTA, pH9.0, 45min. Secondary: HRP-polymer, 30min. DAB, 5min.
TTF-1 / NKX2-1 Antibody (TTF1/8123R)

TTF-1 / NKX2-1 Antibody (TTF1/8123R)

Formalin-fixed, paraffin-embedded human lung stained with TTF-1 / NKX2-1 antibody (TTF1/8123R). Inset: PBS instead of primary antibody; secondary only negative control.

Applications for TTF-1 / NKX2-1 Antibody (TTF1/8123R)

Application
Recommended Usage

Immunohistochemistry-Paraffin

1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes. at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.

Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: TTF-1/NKX2-1

The thyroid transcription factor 1 (TTF-1) is a homeodomain-containing transcription factor that activates the transcriptional activity of thyroid-specific gene promoters by binding to them. Hence, TTF-1 is crucial in the maintenance of the thyroid differentiation phenotype (1). Thyroid dysgenesis (TD) is responsible for most cases of congenital hypothyroidism, a condition that affects about one in 4000 newborns. Mutations in PAX8, TTF1, or FOXE1 may account for congenital hypothyroidism in patients with either isolated TD or TD with associated malformations involving kidney, lung, forebrain, and palate (2). In addition to its presence in thyroid gland epithelium, the human TTF-1 protein was detected by immunohistochemistry in human fetal lung as early as 11 weeks of gestation, being localized in the nuclei of epithelial cells of the developing airways. After birth, TTF-1 was selectively expressed in Type II epithelial cells in the alveoli and in subsets of bronchiolar epithelial cells in the conducting regions of the lung (3).

Long Name

NK2 Homeobox 1

Alternate Names

BCH, BHC, NKX2-1, NKX2.1, NKX2A, T/EBP, TITF1, TTF1

Gene Symbol

NKX2-1

UniProt

P43699

Additional TTF-1/NKX2-1 Products

Product Documents for TTF-1 / NKX2-1 Antibody (TTF1/8123R)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for TTF-1 / NKX2-1 Antibody (TTF1/8123R)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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