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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Porcine

Cited:

Human

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # GCP2-01

Format

BSA Free

Concentration

1.0 mg/ml

Product Specifications

Immunogen

GST-fusion protein containing amino acids 2-194 of mouse GCP2.

Reactivity Notes

Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-questions.

Specificity

The mouse monoclonal antibody GCP2-01 recognizes gamma-tubulin complex component 2 (GCP2), a 95-103 kDa protein. It recognizes also phosphorylated GCP2.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Theoretical MW

103 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for TUBGCP2 Antibody (GCP2-01) - BSA Free

TUBGCP2 Antibody (GCP2-01) - BSA Free Western Blot: TUBGCP2 Antibody (GCP2-01) - BSA Free [NBP2-21793]

Western Blot: TUBGCP2 Antibody (GCP2-01) - BSA Free [NBP2-21793]

Analysis of human gamma-tubulin complex component 2 (GCP2) using mouse monoclonal antibody GCP2-01 on lysates of various cell lines under reducing and non-reducing conditions. Nitrocellulose membrane was probed with 2 µg/ml of mouse anti-GCP2 monoclonal antibody followed by IRDye800-conjugated anti-mouse secondary antibody. A specific band was detected for GCP2 at approximately 95 kDa.

Applications for TUBGCP2 Antibody (GCP2-01) - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50-1:300

Immunohistochemistry

1:50-1:300

Immunohistochemistry-Paraffin

1:10-1:500

Immunoprecipitation

1:10-1:500

Western Blot

1:500-1:2000
Application Notes
Western Blot: can be used, but not optimal

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.4)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: TUBGCP2

Gamma tubulin complex component 2 (GCP2) is 95-103 kDa protein which associates with two molecules of gamma tubulin and one molecule of gamma tubulin complex component 3 (GCP3), to form the gamma tubulin small complexes (gammaTuSCs). These complexes interact with GCP4, 5, and 6 to form the gamma tubulin ring complexes (gammaTuRCs), which are embedded in the microtubule organizing center matrix where they nucleate microtubules. The gammaTuRCs are also involved in the regulation of microtubule plus and minus end dynamics. Components of gamma tubulin complexes are highly conservative in eukaryotes.

Alternate Names

Gamma-ring complex protein 103 kDa, gamma-tubulin complex component 2, gamma-tubulin complex protein 2, GCP-2, GCP2hGrip103, h103p, hGCP2, hSpc97, MGC138162, SPBC97, Spc97p, Spindle pole body protein Spc97 homolog, tubulin, gamma complex associated protein 2

Entrez Gene IDs

10844 (Human)

Gene Symbol

TUBGCP2

Additional TUBGCP2 Products

Product Documents for TUBGCP2 Antibody (GCP2-01) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for TUBGCP2 Antibody (GCP2-01) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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