Tubulin alpha-1B Antibody (114)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-43837
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat, Drosophila, Fish, Goat, Zebrafish
Predicted:
Bovine (100%), Porcine (100%), Primate (99%), Rabbit (100%), Rhesus Macaque (100%), Xenopus (95%). Backed by our 100% Guarantee.
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Tumorsphere, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # 114
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant protein encompassing a sequence within the center region of human Tubulin alpha-1B. The exact sequence is proprietary.
Reactivity Notes
Chimpanzee (99%), Xenopus laevis (95%).
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Theoretical MW
50 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Tubulin alpha-1B Antibody (114)
Immunocytochemistry/ Immunofluorescence: Tubulin alpha-1B Antibody (114) [NBP2-43837]
Immunocytochemistry/Immunofluorescence: Tubulin alpha-1B Antibody (114) [NBP2-43837] - Tubulin alpha-1B Antibody (114) detects Tubulin alpha-1B protein at cytoskeleton by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: alpha Tubulin stained by Tubulin alpha-1B Antibody (114) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar= 10 um.Immunohistochemistry-Paraffin: Tubulin alpha-1B Antibody (114) [NBP2-43837]
Immunohistochemistry-Paraffin: Tubulin alpha-1B Antibody (114) [NBP2-43837] - Paraffin-embedded mouse colon. Red: alpha Tubulin antibody [114] diluted at 1:500. Green: beta Catenin antibody [N1N2-2] (NBP1-32239) diluted at 1:500. Blue: Hoechst 33342 staining.Western Blot: Tubulin alpha-1B Antibody (114) [NBP2-43837]
Western Blot: Tubulin alpha-1B Antibody (114) [NBP2-43837] - Analysis of A. 30 ug 293T whole cell lysate/extract B. 30 ug NIH-3T3 whole cell lysate/extract C. 30 ug mouse brain lysate/extract D. 30 ug PC-12 whole cell lysate/extract E. 30 ug rat brain lysate/extract F. 30 ug drosophila lysae/extract 10 % SDS-PAGE alpha Tubulin antibody [114] dilution: 1:5000.Applications for Tubulin alpha-1B Antibody (114)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100-1:1000
Immunohistochemistry
1:100-1:1000
Immunohistochemistry-Frozen
1:100-1:1000
Immunohistochemistry-Paraffin
1:100-1:1000
Western Blot
1:500-1:20000
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS, 20% Glycerol (pH7)
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: Tubulin alpha-1B
Long Name
Tubulin alpha-1B chain
Alternate Names
TUBA1B, Tubulin K-alpha-1
Gene Symbol
TUBA1B
Additional Tubulin alpha-1B Products
Product Documents for Tubulin alpha-1B Antibody (114)
Product Specific Notices for Tubulin alpha-1B Antibody (114)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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