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Tubulin alpha-1B Antibody (114)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-43837

Novus Biologicals, part of Bio-Techne
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NBP2-43837

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Drosophila, Fish, Goat, Zebrafish

Predicted:

Bovine (100%), Porcine (100%), Primate (99%), Rabbit (100%), Rhesus Macaque (100%), Xenopus (95%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Tumorsphere, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 114

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human Tubulin alpha-1B. The exact sequence is proprietary.

Reactivity Notes

Chimpanzee (99%), Xenopus laevis (95%).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

50 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Tubulin alpha-1B Antibody (114)

Immunocytochemistry/ Immunofluorescence: Tubulin alpha-1B Antibody (114) [NBP2-43837]

Immunocytochemistry/ Immunofluorescence: Tubulin alpha-1B Antibody (114) [NBP2-43837]

Immunocytochemistry/Immunofluorescence: Tubulin alpha-1B Antibody (114) [NBP2-43837] - Tubulin alpha-1B Antibody (114) detects Tubulin alpha-1B protein at cytoskeleton by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: alpha Tubulin stained by Tubulin alpha-1B Antibody (114) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar= 10 um.
Immunohistochemistry-Paraffin: Tubulin alpha-1B Antibody (114) [NBP2-43837]

Immunohistochemistry-Paraffin: Tubulin alpha-1B Antibody (114) [NBP2-43837]

Immunohistochemistry-Paraffin: Tubulin alpha-1B Antibody (114) [NBP2-43837] - Paraffin-embedded mouse colon. Red: alpha Tubulin antibody [114] diluted at 1:500. Green: beta Catenin antibody [N1N2-2] (NBP1-32239) diluted at 1:500. Blue: Hoechst 33342 staining.
Western Blot: Tubulin alpha-1B Antibody (114) [NBP2-43837]

Western Blot: Tubulin alpha-1B Antibody (114) [NBP2-43837]

Western Blot: Tubulin alpha-1B Antibody (114) [NBP2-43837] - Analysis of A. 30 ug 293T whole cell lysate/extract B. 30 ug NIH-3T3 whole cell lysate/extract C. 30 ug mouse brain lysate/extract D. 30 ug PC-12 whole cell lysate/extract E. 30 ug rat brain lysate/extract F. 30 ug drosophila lysae/extract 10 % SDS-PAGE alpha Tubulin antibody [114] dilution: 1:5000.

Applications for Tubulin alpha-1B Antibody (114)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Frozen

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:20000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS, 20% Glycerol (pH7)

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Tubulin alpha-1B

Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain

Long Name

Tubulin alpha-1B chain

Alternate Names

TUBA1B, Tubulin K-alpha-1

Gene Symbol

TUBA1B

Additional Tubulin alpha-1B Products

Product Documents for Tubulin alpha-1B Antibody (114)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Tubulin alpha-1B Antibody (114)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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