Tubulin alpha-3E Antibody (OTI2A8)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-02878

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # OTI2A8

Concentration

1 mg/ml

View all Tubulin alpha-3E Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human TUBA3E (NP_997195) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

49.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Tubulin alpha-3E Antibody (OTI2A8)

Western Blot: Tubulin alpha-3E Antibody (OTI2A8) [NBP2-02878]

Western Blot: Tubulin alpha-3E Antibody (OTI2A8) [NBP2-02878]

Western Blot: Tubulin alpha-3E Antibody (2A8) [NBP2-02878] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Tubulin alpha-3E (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Tubulin alpha-3E.
Immunocytochemistry/Immunofluorescence: Tubulin alpha-3E Antibody (2A8) [NBP2-02878] Staining of COS7 cells transiently transfected by pCMV6-ENTRY Tubulin alpha-3E.
Flow Cytometry: Tubulin alpha-3E Antibody (OTI2A8) [NBP2-02878]

Flow Cytometry: Tubulin alpha-3E Antibody (OTI2A8) [NBP2-02878]

Flow Cytometry: Tubulin alpha-3E Antibody (2A8) [NBP2-02878] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-Tubulin alpha-3E antibody, and then analyzed by flow cytometry.

Applications for Tubulin alpha-3E Antibody (OTI2A8)

Application
Recommended Usage

Flow Cytometry

1:100

Immunocytochemistry/ Immunofluorescence

1:100

Western Blot

1:2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Tubulin alpha-3E

Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain (By similarity)

Long Name

Tubulin alpha-3E chain

Alternate Names

TUBA3E

Entrez Gene IDs

112714 (Human)

Gene Symbol

TUBA3E

Additional Tubulin alpha-3E Products

Product Documents for Tubulin alpha-3E Antibody (OTI2A8)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Tubulin alpha-3E Antibody (OTI2A8)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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