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Tubulin alpha-8 Antibody (OTI2G6)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-02474

Novus Biologicals, part of Bio-Techne
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NBP2-02474
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Canine, Monkey

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # OTI2G6

Concentration

0.82 mg/ml

Product Summary for Tubulin alpha-8 Antibody (OTI2G6)

Immunogen

Full length human recombinant protein of human TUBA8(NP_061816) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

50.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Tubulin alpha-8 Antibody (OTI2G6)

Western Blot: Tubulin alpha-8 Antibody (OTI2G6) [NBP2-02474]

Western Blot: Tubulin alpha-8 Antibody (OTI2G6) [NBP2-02474]

Western Blot: Tubulin alpha-8 Antibody (OTI2G6) [NBP2-02474] - WB analysis of extracts (35ug) from 9 different cell lines by using anti-Tubulin alpha-8 monoclonal antibody.
Immunocytochemistry/ Immunofluorescence: Tubulin alpha-8 Antibody (OTI2G6) [NBP2-02474]

Immunocytochemistry/ Immunofluorescence: Tubulin alpha-8 Antibody (OTI2G6) [NBP2-02474]

Immunocytochemistry/Immunofluorescence: Tubulin alpha-8 Antibody (OTI2G6) [NBP2-02474] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Tubulin alpha-8.
Immunohistochemistry-Paraffin: Tubulin alpha-8 Antibody (OTI2G6) [NBP2-02474]

Immunohistochemistry-Paraffin: Tubulin alpha-8 Antibody (OTI2G6) [NBP2-02474]

Immunohistochemistry-Paraffin: Tubulin alpha-8 Antibody (OTI2G6) [NBP2-02474] - Staining of paraffin-embedded prostate tissue using anti-Tubulin alpha-8 mouse monoclonal antibody.

Applications for Tubulin alpha-8 Antibody (OTI2G6)

Application
Recommended Usage

Flow Cytometry

1:100

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:50-1:150

Immunohistochemistry-Paraffin

1:50

Western Blot

1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

0.82 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Tubulin alpha-8

Microtubules are cylindrical tubes of 20-25 nm in diameter. They are composed of protofilaments which are in turn composed of alpha- and beta-tubulin polymers. Each microtubule is polarized, at one end alpha-subunits are exposed (-) and at the other beta-subunits are exposed (+). Microtubules act as a scaffold to determine cell shape, and provide a backbone for cell organelles and vesicles to move on, a process that requires motor proteins. The major microtubule motor proteins are kinesin, which generally moves towards the (+) end of the microtubule, and dynein, which generally moves towards the (-) end. Microtubules also form the spindle fibers for separating chromosomes during mitosis.

Long Name

Tubulin alpha-8 chain

Alternate Names

Alpha-tubulin 8, TUBA8, TUBAL2

Entrez Gene IDs

51807 (Human)

Gene Symbol

TUBA8

Additional Tubulin alpha-8 Products

Product Documents for Tubulin alpha-8 Antibody (OTI2G6)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Tubulin alpha-8 Antibody (OTI2G6)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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