Tubulin Beta 4 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-32205
![Immunocytochemistry/ Immunofluorescence: Tubulin Beta 4 Antibody [NBP1-32205]](https://resources.bio-techne.com/images/products/Tubulin-Beta-4-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-32205-img0002.jpg "Immunocytochemistry/ Immunofluorescence: Tubulin Beta 4 Antibody [NBP1-32205]")
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Predicted:
Bovine (100%), Chimpanzee (100%), Xenopus (96%). Backed by our 100% Guarantee.
Applications
Validated:
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot
Cited:
IF/IHC
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant protein encompassing a sequence within the center region of human Tubulin Beta 4. The exact sequence is proprietary.
Reactivity Notes
Xenopus laevis (96%).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
50 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Tubulin Beta 4 Antibody
![Western Blot: Tubulin Beta 4 Antibody [NBP1-32205]](https://resources.bio-techne.com/images/products/Tubulin-Beta-4-Antibody-Western-Blot-NBP1-32205-img0004.jpg "Western Blot: Tubulin Beta 4 Antibody [NBP1-32205]")
Western Blot: Tubulin Beta 4 Antibody [NBP1-32205]
Western Blot: Tubulin Beta 4 Antibody [NBP1-32205] - Sample(30 ug of whole cell lysate) A:293T7. 5% SDS PAGE, antibody diluted at 1:1000.![Immunocytochemistry/ Immunofluorescence: Tubulin Beta 4 Antibody [NBP1-32205]](https://resources.bio-techne.com/images/products/Tubulin-Beta-4-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-32205-img0005.jpg "Immunocytochemistry/ Immunofluorescence: Tubulin Beta 4 Antibody [NBP1-32205]")
Immunocytochemistry/ Immunofluorescence: Tubulin Beta 4 Antibody [NBP1-32205]
Immunocytochemistry/Immunofluorescence: Tubulin Beta 4 Antibody [NBP1-32205] - Tubulin Beta 4 Antibody detects Tubulin Beta 4 protein by immunofluorescent analysis. Sample: DIV9 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Tubulin Beta 4 stained by Tubulin Beta 4 Antibody diluted at 1:500. Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody diluted at 1:500. Blue: Fluoroshield with DAPI.![Western Blot: Tubulin Beta 4 Antibody [NBP1-32205]](https://resources.bio-techne.com/images/products/Tubulin-Beta-4-Antibody-Western-Blot-NBP1-32205-img0003.jpg "Western Blot: Tubulin Beta 4 Antibody [NBP1-32205]")
Western Blot: Tubulin Beta 4 Antibody [NBP1-32205]
Western Blot: Tubulin Beta 4 Antibody [NBP1-32205] - Sample (30 ug of whole cell lysate) A: NIH-3T3 10% SDS PAGE; antibody diluted at 1:1000.Applications for Tubulin Beta 4 Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100-1:1000
Immunohistochemistry
1:100-1:1000
Western Blot
1:500-1:3000
Application Notes
IHC usage reported in scientific literature (PMID: 24624341).
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
0.1M Tris (pH 7), 0.1M Glycine, 10% Glycerol
Preservative
0.01% Thimerosal
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: Tubulin Beta 4
Long Name
Tubulin beta-4A chain
Alternate Names
TUBB4, TUBB4A, Tubulin 5 beta
Gene Symbol
TUBB4A
Additional Tubulin Beta 4 Products
Product Documents for Tubulin Beta 4 Antibody
Product Specific Notices for Tubulin Beta 4 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information go to www.P65Warnings.ca.gov.